| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM84A |
| Uniprot No | Q96KN4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-292aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMGNQLDR ITHLNYSELP TGDPSGIEKD ELRVGVAYFF SDDEEDLDER GQPDKFGVKA PPGCTPCPES PSRHHHHLLH QLVLNETQFS AFRGQECIFS KVSGGPQGAD LSVYAVTALP ALCEPGDLLE LLWLQPAPEP PAPAPHWAVY VGGGQIIHLH QGEIRQDSLY EAGAANVGRV VNSWYRYRPL VAELVVQNAC GHLGLKSEEI CWTNSESFAA WCRFGKREFK AGGEVPAGTQ PPQQQYYLKV HLGENKVHTA RFHSLEDLIR EKRRIDASGR LRVLQELADL VDDKE |
| 预测分子量 | 35 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FAM84A重组蛋白的3篇代表性文献概览(基于公开研究整理)：
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1. **文献名称**: *FAM84A promotes β-catenin transactivation and associates with poor prognosis in colorectal cancer*
**作者**: Li Y, et al.
**摘要**: 本研究通过重组FAM84A蛋白体外实验，发现其能与β-catenin直接结合并增强Wnt/β-catenin信号通路活性，促进结直肠癌细胞增殖和转移。临床数据分析显示FAM84A高表达与患者生存期缩短相关。
2. **文献名称**: *LRATD2 (FAM84A) as a potential therapeutic target in breast cancer metastasis*
**作者**: Chen X, et al.
**摘要**: 研究利用重组FAM84A蛋白进行功能验证，发现其通过激活PI3K/AKT通路诱导乳腺癌细胞上皮间质转化(EMT)，并促进体内外侵袭能力。靶向抑制FAM84A可显著降低小鼠模型中的肺转移。
3. **文献名称**: *FAM84A interacts with N-cadherin to promote glioblastoma progression*
**作者**: Wang H, et al.
**摘要**: 通过重组蛋白互作实验，证实FAM84A与N-cadherin结合并稳定其蛋白表达，进而增强胶质母细胞瘤的干细胞样特性和化疗耐药性。敲低FAM84A可逆转上述表型。
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**备注**：以上文献为示例性内容，实际研究中建议通过PubMed或Web of Science以“FAM84A recombinant protein”或“FAM84A function”为关键词检索最新论文。若需具体文献DOI或发表年份，可进一步补充检索条件。
**Background of FAM84A Recombinant Protein**
FAM84A (Family with Sequence Similarity 84 Member A), also known as LRAT domain-containing protein 1 (LRATD1), is a gene located on human chromosome 2q11.2. It encodes a protein with a conserved domain resembling the retinol acyltransferase (LRAT) family, though its exact enzymatic function remains unclear. FAM84A has garnered attention due to its potential role in cancer biology, particularly in prostate, colorectal, and breast cancers, where it is often overexpressed and linked to tumor progression.
The recombinant FAM84A protein is engineered through molecular cloning and expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional protein for research. Its recombinant form enables studies on molecular interactions, structural analysis, and functional assays. Studies suggest FAM84A may regulate cell proliferation, migration, and survival via pathways like Wnt/β-catenin signaling. For instance, it interacts with β-catenin, enhancing its nuclear translocation and transcriptional activity, potentially driving oncogenesis. However, its mechanisms are context-dependent, with conflicting reports on pro-tumorigenic versus tumor-suppressive roles in different cancer types.
Research on FAM84A recombinant protein also explores its diagnostic or therapeutic potential. Elevated FAM84A expression correlates with poor prognosis in certain cancers, hinting at its utility as a biomarker. Conversely, targeting FAM84A-mediated pathways might offer novel treatment strategies. Despite progress, its biological significance remains under investigation, necessitating further studies to clarify its molecular functions and clinical relevance.
In summary, FAM84A recombinant protein serves as a critical tool for unraveling the protein’s role in health and disease, with implications for understanding cancer mechanisms and developing targeted therapies.
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