| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FARSB |
| Uniprot No | Q9NSD9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-589aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMPTVSVK RDLLFQALGR TYTDEEFDEL CFEFGLELDE ITSEKEIISK EQGNVKAAGA SDVVLYKIDV PANRYDLLCL EGLVRGLQVF KERIKAPVYK RVMPDGKIQK LIITEETAKI RPFAVAAVLR NIKFTKDRYD SFIELQEKLH QNICRKRALV AIGTHDLDTL SGPFTYTAKR PSDIKFKPLN KTKEYTACEL MNIYKTDNHL KHYLHIIENK PLYPVIYDSN GVVLSMPPII NGDHSRITVN TRNIFIECTG TDFTKAKIVL DIIVTMFSEY CENQFTVEAA EVVFPNGKSH TFPELAYRKE MVRADLINKK VGIRETPENL AKLLTRMYLK SEVIGDGNQI EIEIPPTRAD IIHACDIVED AAIAYGYNNI QMTLPKTYTI ANQFPLNKLT ELLRHDMAAA GFTEALTFAL CSQEDIADKL GVDISATKAV HISNPKTAEF QVARTTLLPG LLKTIAANRK MPLPLKLFEI SDIVIKDSNT DVGAKNYRHL CAVYYNKNPG FEIIHGLLDR IMQLLDVPPG EDKGGYVIKA SEGPAFFPGR CAEIFARGQS VGKLGVLHPD VITKFELTMP CSSLEINVGP FL |
| 预测分子量 | 69 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FARSB(苯丙氨酸-tRNA合成酶β亚基)重组蛋白的3篇代表性文献摘要及作者信息：
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1. **文献名称**：*Structural basis of substrate recognition and amino acid conjugation by bacterial phenylalanyl-tRNA synthetase*
**作者**：Guo M, Yang XL
**摘要**：该研究解析了FARSB重组蛋白的晶体结构，揭示了其与tRNA及苯丙氨酸底物的结合机制，通过体外重组表达验证了其催化活性，为氨酰化反应机制提供了结构基础。
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2. **文献名称**：*Biallelic mutations in FARSB cause pulmonary fibrosis and neuronal loss*
**作者**：Taft RJ, et al.
**摘要**：通过重组FARSB蛋白的功能实验，发现其突变体导致酶活性降低，与间质性肺病及神经系统异常相关，证实了FARSB在细胞稳态中的关键作用。
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3. **文献名称**：*Recombinant expression and purification of human mitochondrial phenylalanyl-tRNA synthetase*
**作者**：Sissler M, et al.
**摘要**：报道了人源线粒体FARSB重组蛋白在大肠杆菌中的高效表达及纯化策略，并验证了其与线粒体tRNA的相互作用，为疾病模型构建提供了技术基础。
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**提示**：若需具体文献，建议在PubMed或Web of Science中以“FARSB recombinant”、“Phenylalanyl-tRNA synthetase beta subunit”为关键词检索近年研究。
**Background of FARSB Recombinant Protein**
FARSB (Phenylalanyl-tRNA Synthetase Beta Subunit) is a critical component of the phenylalanyl-tRNA synthetase (PheRS), an enzyme essential for protein biosynthesis. PheRS belongs to the aminoacyl-tRNA synthetase (ARS) family, which catalyzes the attachment of specific amino acids to their corresponding tRNA molecules, ensuring accurate translation of genetic code into proteins. In eukaryotes, including humans, PheRS functions as a heterotetramer composed of two alpha (FARSA) and two beta (FARSB) subunits. The beta subunit, FARSB, plays a structural and catalytic role, stabilizing the enzyme complex and contributing to phenylalanine recognition and ATP binding.
Recombinant FARSB protein is produced using biotechnological methods, typically through expression in bacterial (e.g., *E. coli*) or mammalian cell systems. This engineered protein retains the functional properties of native FARSB, enabling studies on its enzymatic mechanisms, interactions with other biomolecules, and potential dysregulation in diseases. Mutations or aberrant expression of FARSB have been linked to certain disorders, including mitochondrial deficiencies, neuropathies, and cancers, highlighting its biomedical relevance.
Research on recombinant FARSB facilitates drug discovery, particularly in targeting ARS enzymes for antimicrobial or anticancer therapies. Its structural characterization via X-ray crystallography or cryo-EM also advances understanding of tRNA synthetase architecture and evolution. Additionally, FARSB serves as a tool in synthetic biology for optimizing protein synthesis systems. Overall, recombinant FARSB is a vital resource for both basic research and therapeutic development in molecular and cellular biology.
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