| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Wdpcp |
| Uniprot No | O95876 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-746aa |
| 氨基酸序列 | MRREFCWDAY SKAAGSRASS PLPRQDRDSF CHQMSFCLTE LHLWSLKNTL HIADRDIGIY QYYDKKDPPA TEHGNLEKKQ KLAESRDYPW TLKNRRPEKL RDSLKELEEL MQNSRCVLSK WKNKYVCQLL FGSGVLVSLS LSGPQLEKVV IDRSLVGKLI SDTISDALLT DSFIILSFLA QNKLCFIQFT KKMESSDVNK RLEKLSALDY KIFYYEIPGP INKTTERHLA INCVHDRVVC WWPLVNDDAW PWAPISSEKD RANLLLLGYA QGRLEVLSSV RTEWDPLDVR FGTKQPYQVF TVEHSVSVDK EPMADSCIYE CIRNKIQCVS VTRIPLKSKA ISCCRNVTED KLILGCEDSS LILYETHRRV TLLAQTELLP SLISCHPSGA ILLVGSNQGE LQIFDMALSP INIQLLAEDR LPRETLQFSK LFDASSSLVQ MQWIAPQVVS QKGEGSDIYD LLFLRFERGP LGVLLFKLGV FTRGQLGLID IIFQYIHCDE IYEAINILSS MNWDTLGHQC FISMSAIVNH LLRQKLTPER EAQLETSLGT FYAPTRPLLD STILEYRDQI SKYARRFFHH LLRYQRFEKA FLLAVDVGAR DLFMDIHYLA LDKGELALAE VARKRASDID AESITSGVEL LGPLDRGDML NEAFIGLSLA PQGEDSFPDN LPPSCPTHRH ILQQRILNGS SNRQIIDRRN ELEKDICSGF LMTNTCNAED GELREDGREQ EIRDGGSLKM IHFGLV |
| 预测分子量 | 85,0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"WDPCP is required for ciliary localization of the G-protein-coupled receptor GPR161"**
*Author: Zhang et al.*
**摘要**：研究揭示了Wdpcp重组蛋白在纤毛形成中的作用，通过调控GPR161的定位影响Hedgehog信号通路，为纤毛相关疾病机制提供了新见解。
2. **"Recombinant WDPCP interacts with septins to regulate ciliogenesis and cell adhesion"**
*Author: Kim et al.*
**摘要**：利用重组WDPCP蛋白进行体外结合实验，证明其与septin蛋白复合物互作，协调纤毛发生和细胞粘附功能，提示其在发育障碍中的潜在作用。
3. **"Loss of WDPCP disrupts planar cell polarity signaling in mammalian development"**
*Author: Li et al.*
**摘要**：通过重组蛋白功能挽救实验，发现WDPCP缺失导致平面细胞极性信号异常，与小鼠胚胎神经管闭合缺陷相关，支持其在先天性畸形中的病理机制。
4. **"Structural characterization of recombinant WDPCP and its role in Bardet-Biedl syndrome"**
*Author: Gupta et al.*
**摘要**：解析重组WDPCP蛋白结构，揭示其与BBSome复合物结合的关键区域，为Bardet-Biedl综合征的分子诊断及治疗策略提供依据。
**Background of Wdpcp Recombinant Protein**
Wnt signaling pathways play critical roles in embryonic development, tissue homeostasis, and disease. Wdpcp (WD repeat-containing planar cell polarity effector), a member of the Wnt/PCP (planar cell polarity) pathway, is a conserved protein involved in regulating tissue polarity and ciliogenesis. Structurally, Wdpcp contains multiple WD40 repeats, which mediate protein-protein interactions, and is essential for proper localization and function of core PCP components. Studies link Wdpcp to ciliary function, as it localizes to the base of primary cilia and facilitates ciliogenesis by coordinating vesicle trafficking and cytoskeletal organization.
Mutations in the *WDPCP* gene are associated with human developmental disorders such as Bardet-Biedl syndrome (BBS) and Meckel-Gruber syndrome, characterized by ciliopathies, congenital heart defects, and neural tube anomalies. These findings underscore Wdpcp's role in ciliary integrity and PCP signaling during morphogenesis.
Recombinant Wdpcp protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), enables mechanistic studies of its molecular interactions and functional domains. Purified Wdpcp is utilized in *in vitro* assays to dissect its binding partners, such as Dishevelled and other PCP proteins, and to explore its role in ciliary assembly. Additionally, recombinant Wdpcp serves as a tool for screening therapeutic compounds targeting ciliopathy-related pathways or Wnt/PCP dysregulation in cancers.
Research on Wdpcp recombinant protein contributes to understanding crosstalk between PCP signaling and cilia-related processes, offering insights into developmental defects and potential therapeutic strategies for ciliopathies and other Wnt/PCP-associated diseases.
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