| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DYDC2 |
| Uniprot No | Q96IM9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-177aa |
| 氨基酸序列 | METNYLKRCFGNCLAQALAEVAKVRPSDPIEYLAHWLYHYRKTAKAKEENREKKIHLQEEYDSSLKEMEMTEMLKQEEYQIQQNCEKCHKELTSETVSTKKTIFMQEDTNPLEKEALKQEFLPGTSSLIPGMPQQVPPSESAGQIDQNFKMPQEINYKEAFQHEVAHEMPPGSKSPF |
| 预测分子量 | 36.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
关于DYDC2重组蛋白的研究文献目前公开可查的较少，以下为模拟的示例性参考文献(非真实存在，仅供格式参考)：
1. **文献名称**：Structural Characterization of DYDC2 Recombinant Protein in Cancer Signaling
**作者**：Li X, et al.
**摘要**：研究通过大肠杆菌表达系统纯化了DYDC2重组蛋白，解析了其晶体结构，并发现其通过调控Wnt/β-catenin通路影响肿瘤细胞迁移。
2. **文献名称**：DYDC2 Recombinant Protein Promotes Neuronal Differentiation
**作者**：Wang Y, et al.
**摘要**：利用哺乳动物细胞表达的DYDC2重组蛋白，证实其通过激活MAPK通路促进神经干细胞向神经元分化，为神经再生研究提供新靶点。
3. **文献名称**：Functional Analysis of DYDC2 in Viral Infection
**作者**：Zhang R, et al.
**摘要**：通过昆虫细胞系统制备DYDC2重组蛋白，发现其能与特定病毒衣壳蛋白结合，抑制病毒入侵宿主细胞的早期步骤。
**提示**：若需真实文献，建议：
1. 核查基因名称准确性(DYDC2是否为正确命名，或存在拼写差异);
2. 在PubMed或Google Scholar中使用关键词组合检索(如：DYDC2 recombinant protein, DYDC2 expression purification);
3. 关注近年蛋白质数据库(PDB)中可能存在的结构解析记录。
DYDC2 (Dpy-30 domain-containing protein 2) is a eukaryotic protein belonging to the Dpy-30 family, which is evolutionarily conserved from yeast to humans. It is characterized by a conserved Dpy-30 domain, a structural motif implicated in mediating protein-protein interactions, particularly within chromatin-modifying complexes. DYDC2 is closely associated with the regulation of histone methylation, a key epigenetic mechanism influencing gene expression. Studies suggest its involvement in the activity of the COMPASS (Complex of Proteins Associated with Set1)-like complexes, which catalyze histone H3 lysine 4 (H3K4) methylation—a hallmark of transcriptionally active chromatin.
Recombinant DYDC2 protein is engineered using genetic cloning techniques, typically expressed in bacterial or mammalian systems to ensure proper folding and post-translational modifications. The recombinant form retains the functional Dpy-30 domain, enabling researchers to study its biochemical interactions, structural dynamics, and regulatory roles in vitro. Its production often includes affinity tags (e.g., His-tag) for simplified purification and detection.
Functionally, DYDC2 is proposed to act as an adaptor protein, facilitating the recruitment or stabilization of histone methyltransferase complexes at specific genomic loci. Dysregulation of DYDC2 has been linked to developmental abnormalities and diseases, including cancers, where aberrant histone methylation patterns contribute to oncogenesis. Current research focuses on elucidating its precise molecular mechanisms, exploring its potential as a therapeutic target, and leveraging recombinant DYDC2 in high-throughput screens for epigenetic drug discovery. Its study also advances understanding of chromatin remodeling, cellular differentiation, and transcriptional regulation in health and disease.
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