| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PIK3R5 |
| Uniprot No | Q8WYR1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-494aa |
| 氨基酸序列 | MDSGYVEDSEESSSEWPWRRGSQERRGHRRPGQKFIRIYKLFKSTSQLVLRRDSRSLEGSSDTALPLRRAGSLCSPLDEPVSPPSRAQRSRSLPQPKLGTQLPSWLLAPASRPQRRRPFLSGDEDPKASTLRVVVFGSDRISGKVARAYSNLRRLENNRPLLTRFFKLQFFYVPVKRSHGTSPGACPPPRSQTPSPPTDSPRHASPGELGTTPWEESTNDISHYLGMLDPWYERNVLGLMHLPPEVLCQQSLKAEAQALEGSPTQLPILADMLLYYCRFAARPVLLQVYQTELTFITGEKTTEIFIHSLELGHSAATRAIKASGPGSKRLGIDGDREAVPLTLQIIYSKGAISGRSRWSNLEKVCTSVNLNKACRKQEELDSSMEALTLNLTEVVKRQNSKSKKGFNQISTSQIKVDKVQIIGSNSCPFAVCLDQDERKILQSVVRCEVSPCYKPEKSDLSSPPQTPPDLPAQAAPDLCSLLCLPIMTFSGALP |
| 预测分子量 | 70.9kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PIK3R5重组蛋白的假设性参考文献示例(注：文献为模拟生成，非真实存在)：
1. **文献名称**：*Structural Insights into the Regulatory Role of Recombinant PIK3R5 in PI3K Signaling*
**作者**：Smith J, Brown K, Zhang Y
**摘要**：本研究通过表达并纯化重组人源PIK3R5蛋白，结合晶体结构分析，揭示了其与p110γ催化亚基的相互作用机制，阐明其在PI3K-AKT信号通路中的调控功能，为靶向PI3K的癌症治疗提供结构基础。
2. **文献名称**：*Optimized Expression and Functional Characterization of Human PIK3R5 in a Mammalian System*
**作者**：Lee H, Park S, Kim T
**摘要**：开发了一种在HEK293细胞中高效表达重组PIK3R5蛋白的方法，并通过体外激酶实验证明其对PI3K活性的抑制作用，探讨了其在胰岛素信号传导中的潜在作用。
3. **文献名称**：*PIK3R5 Recombinant Protein Modulates Macrophage Polarization via PI3K Pathway*
**作者**：Chen R, Wang L, Liu X
**摘要**：利用重组PIK3R5蛋白处理巨噬细胞，发现其通过抑制PI3K通路促进M1型极化，提示其在炎症性疾病中的调控作用，为免疫治疗研究提供新方向。
4. **文献名称**：*Development of a High-Yield Purification Protocol for Recombinant PIK3R5 and Its Application in Drug Screening*
**作者**：Garcia M, Patel D, Nguyen Q
**摘要**：报道了一种基于亲和层析的高效纯化重组PIK3R5蛋白方案，并利用该蛋白筛选小分子抑制剂，鉴定出两种化合物可特异性阻断其与p110δ亚基的结合。
**提示**：以上文献为示例，实际研究中请通过PubMed、Web of Science或专业数据库检索真实文献。
**Background of PIK3R5 Recombinant Protein**
The **phosphoinositide-3-kinase regulatory subunit 5 (PIK3R5)**, also known as p101. is a regulatory component of the class IB phosphoinositide 3-kinase (PI3K) complex. PI3Ks are lipid kinases that phosphorylate phosphatidylinositol lipids, playing pivotal roles in cellular signaling pathways regulating cell growth, survival, metabolism, and immune responses. PIK3R5 specifically partners with the catalytic subunit p110γ (PIK3CG) to form the PI3Kγ complex, which is primarily expressed in immune cells and regulates inflammatory signaling, chemotaxis, and leukocyte activation.
Recombinant PIK3R5 protein is engineered through molecular cloning and expression systems (e.g., *E. coli*, mammalian cells) to produce purified, functional protein for research. Its recombinant form enables detailed studies of PI3Kγ's structure, activation mechanisms, and interactions with signaling molecules like G-protein-coupled receptors (GPCRs) or RAS family proteins. Dysregulation of PI3Kγ signaling, linked to PIK3R5 dysfunction, is implicated in diseases such as cancer, autoimmune disorders, and chronic inflammation.
Researchers utilize PIK3R5 recombinant protein to investigate its role in immune modulation, screen for inhibitors, or study mutations affecting PI3Kγ activity. Challenges in production include maintaining protein stability and post-translational modifications critical for its function. Structural studies of recombinant PIK3R5-p110γ complexes have advanced drug discovery, particularly in targeting PI3Kγ for anti-inflammatory or anticancer therapies. Overall, PIK3R5 recombinant protein serves as a vital tool for unraveling PI3Kγ biology and its therapeutic potential.
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