| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PHLDA1 |
| Uniprot No | Q8WV24 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-401aa |
| 氨基酸序列 | MRRAPAAERLLELGFPPRCGRQEPPFPLGVTRGWGRWPIQKRREGARPVPFSERSQEDGRGPAARSSGTLWRIRTRLSLCRDPEPPPPLCLLRVSLLCALRAGGRGSRWGEDGARLLLLPPARAAGNGEAEPSGGPSYAGRMLESSGCKALKEGVLEKRSDGLLQLWKKKCCILTEEGLLLIPPKQLQHQQQQQQQQQQQQQQQPGQGPAEPSQPSGPAVASLEPPVKLKELHFSNMKTVDCVERKGKYMYFTVVMAEGKEIDFRCPQDQGWNAEITLQMVQYKNRQAILAVKSTRQKQQHLVQQQPPSQPQPQPQLQPQPQPQPQPQPQPQSQPQPQPQPKPQPQQLHPYPHPHPHPHSHPHSHPHPHPHPHPHQIPHPHPQPHSQPHGHRLLRSTSNSA |
| 预测分子量 | 50.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PHLDA1重组蛋白的3篇参考文献及其摘要概括：
1. **标题**：PHLDA1重组蛋白通过AKT/mTOR通路抑制乳腺癌细胞增殖
**作者**：Zhang Y, Liu X, Chen L
**摘要**：本研究通过构建并纯化人源PHLDA1重组蛋白，发现其能显著抑制乳腺癌细胞MCF-7的增殖并诱导凋亡。机制研究表明，PHLDA1通过下调AKT/mTOR信号通路活性发挥作用，提示其在乳腺癌治疗中的潜在价值。
2. **标题**：PHLDA1重组蛋白减轻肝纤维化的作用及机制
**作者**：Li H, Wang J, Zhou R
**摘要**：研究团队利用原核表达系统制备PHLDA1重组蛋白，并在小鼠肝纤维化模型中验证其功能。结果显示，PHLDA1通过抑制TGF-β/Smad3通路减少肝星状细胞活化，显著降低胶原沉积，为肝纤维化治疗提供新策略。
3. **标题**：PHLDA1重组蛋白调控T细胞活化的分子机制
**作者**：Wang T, Xu S, Chen Q
**摘要**：该研究通过体外表达PHLDA1重组蛋白，发现其能结合T细胞受体复合物中的ZAP-70蛋白，抑制T细胞过度活化。这一发现揭示了PHLDA1在自身免疫性疾病中的免疫调节作用。
4. **标题**：PHLDA1重组蛋白的结构解析及其与脂质结合特性
**作者**：Smith J, Brown K, Lee M
**摘要**：通过X射线晶体学解析了PHLDA1重组蛋白的三维结构，发现其PH结构域特异性结合磷脂酰肌醇-4.5-二磷酸(PIP2)。此结构基础为阐明PHLDA1在细胞膜定位和信号转导中的功能提供依据。
(注：上述文献为示例性内容，实际引用时建议查询最新数据库获取真实文献。)
**Background of PHLDA1 Recombinant Protein**
PHLDA1 (Pleckstrin Homology-like Domain Family A Member 1), also known as TDAG51. is a stress-responsive gene implicated in apoptosis, cell proliferation, and differentiation. It encodes a protein characterized by an N-terminal pleckstrin homology (PH) domain, which facilitates interactions with lipids or proteins, and a C-terminal proline-rich region. PHLDA1 is regulated by diverse stimuli, including DNA damage, oxidative stress, and cytokines, and functions as a modulator of signaling pathways such as Akt/PKB and NF-κB.
Recombinant PHLDA1 protein is produced using biotechnological systems (e.g., *E. coli*, mammalian cells) to enable functional studies. Its recombinant form retains structural and functional integrity, allowing researchers to investigate its role in diseases. For instance, PHLDA1 exhibits dual roles in cancer—acting as a tumor suppressor in some contexts (e.g., colorectal cancer) while promoting survival in others (e.g., breast cancer). It also contributes to pathologies like ischemia-reperfusion injury and diabetes by regulating apoptosis and inflammation.
In research, recombinant PHLDA1 is used to study protein-protein interactions, cellular localization, and downstream signaling. It aids in developing antibodies, screening therapeutic agents, and modeling disease mechanisms. The protein’s purity and activity are ensured via affinity tags (e.g., His-tag) and validation assays (e.g., Western blot, ELISA).
Overall, PHLDA1 recombinant protein serves as a critical tool for unraveling its context-dependent biological roles and exploring therapeutic strategies targeting PHLDA1-associated pathways.
×
