| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TCEANC2 |
| Uniprot No | Q96MN5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-208aa |
| 氨基酸序列 | MDKFVIRTPRIQNSPQKKDSGGKVYKQATIESLKRVVVVEDIKRWKTMLELPDQTKENLVEALQELKKKIPSREVLKSTRIGHTVNKMRKHSDSEVASLAREVYTEWKTFTEKHSNRPSIEVRSDPKTESLRKNAQKLLSEALELKMDHLLVENIERETFHLCSRLINGPYRRTVRALVFTLKHRAEIRAQVKSGSLPVGTFVQTHKK |
| 预测分子量 | 40.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为模拟的示例文献，供参考(请注意实际文献需通过学术数据库检索确认)：
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1. **文献名称**：Functional Characterization of TCEANC2 in DNA Repair Pathways
**作者**：Zhang Y. et al.
**摘要**：研究揭示了TCEANC2重组蛋白在DNA损伤修复中的作用，证实其通过与BRCA1复合物互作调控同源重组修复。体外实验表明，TCEANC2缺失会导致细胞对电离辐射敏感性增加。
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2. **文献名称**：Expression and Purification of Recombinant TCEANC2 in E. coli
**作者**：Lee S. et al.
**摘要**：报道了一种高效的大肠杆菌表达系统，用于可溶性TCEANC2重组蛋白的制备。通过优化诱导条件和纯化步骤，获得高纯度蛋白，并验证其结构完整性。
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3. **文献名称**：TCEANC2 as a Potential Biomarker in Glioblastoma
**作者**：Wang X. et al.
**摘要**：通过临床样本分析，发现TCEANC2在胶质母细胞瘤中异常高表达。重组蛋白功能实验表明，其通过调控Wnt信号通路促进肿瘤细胞侵袭。
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**提示**：
- 若需真实文献，建议使用关键词 **"TCEANC2 recombinant protein"** 在 **PubMed/Google Scholar** 检索。
- 若该蛋白研究较少，可扩展至其基因功能(如染色体定位、互作蛋白)或疾病关联性进行搜索。
希望以上示例对您有帮助!
**Background of TCEANC2 Recombinant Protein**
TCEANC2 (Transcription Elongation Factor A N-Terminal and C-Terminal domain-containing protein 2), also known as TFIIS N-terminal domain-containing protein 2. is a less-studied member of the transcription elongation factor family. It shares structural homology with the transcription elongation factor TFIIS, which plays a critical role in RNA polymerase II-mediated transcription by resolving transcriptional pausing and ensuring efficient RNA synthesis. TCEANC2 is hypothesized to participate in transcriptional regulation, potentially influencing chromatin remodeling or RNA processing, though its precise molecular mechanisms remain unclear.
The recombinant TCEANC2 protein is engineered using heterologous expression systems, such as *E. coli* or mammalian cells, to produce a purified, functional form for experimental studies. This allows researchers to investigate its biochemical properties, interactome, and role in cellular processes. Recombinant TCEANC2 is often tagged with affinity markers (e.g., His-tag, GST) to facilitate purification and detection.
Interest in TCEANC2 stems from its potential involvement in diseases. Preliminary studies suggest links to cancer progression, neurodevelopmental disorders, or viral infection responses, possibly due to its regulatory effects on gene expression. However, these associations require further validation. Current research focuses on elucidating its structure-function relationships, post-translational modifications, and interactions with transcriptional machinery or nucleic acids.
The availability of recombinant TCEANC2 protein accelerates *in vitro* assays, structural studies (e.g., X-ray crystallography), and high-throughput screening for drug discovery. Its characterization may uncover novel therapeutic targets for diseases linked to transcriptional dysregulation. Despite its emerging significance, TCEANC2 remains understudied, highlighting the need for comprehensive functional analyses to define its biological and pathological relevance.
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