| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TSSK3 |
| Uniprot No | Q96PN8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-268aa |
| 氨基酸序列 | MEDFLLSNGYQLGKTIGEGTYSKVKEAFSKKHQRKVAIKVIDKMGGPEEFIQRFLPRELQIVRTLDHKNIIQVYEMLESADGKICLVMELAEGGDVFDCVLNGGPLPESRAKALFRQMVEAIRYCHGCGVAHRDLKCENALLQGFNLKLTDFGFAKVLPKSHRELSQTFCGSTAYAAPEVLQGIPHDSKKGDVWSMGVVLYVMLCASLPFDDTDIPKMLWQQQKGVSFPTHLSISADCQDLLKRLLEPDMILRPSIEEVSWHPWLAST |
| 预测分子量 | 57.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TSSK3重组蛋白的3篇参考文献的简要信息(注：文献为示例性质，实际文献可能需要根据具体数据库检索)：
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1. **文献名称**: "Characterization of the Testis-Specific Serine/Threonine Kinase 3 (TSSK3) Recombinant Protein and Its Role in Sperm Motility"
**作者**: Smith A, et al.
**摘要**: 本研究通过大肠杆菌表达系统成功纯化了重组TSSK3蛋白，并验证了其激酶活性。实验表明，TSSK3在精子尾部蛋白磷酸化中起关键作用，可能影响精子运动能力，为男性不育机制研究提供新方向。
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2. **文献名称**: "Expression and Functional Analysis of TSSK3 in Mammalian Germ Cell Development"
**作者**: Chen L, Wang Y, et al.
**摘要**: 作者利用昆虫细胞-杆状病毒系统表达重组TSSK3蛋白，发现其特异性磷酸化底物蛋白TPX1.体内实验证实TSSK3缺失会导致小鼠精子发生异常，提示其在配子形成中的必要性。
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3. **文献名称**: "Structural Insights into TSSK3 Kinase Domain via Recombinant Protein Crystallography"
**作者**: Johnson R, et al.
**摘要**: 通过重组TSSK3激酶结构域的结晶与结构解析，揭示了其ATP结合口袋的独特构象，为开发靶向TSSK3的小分子抑制剂(如男性避孕药物)提供了结构基础。
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**备注**：以上为模拟文献摘要，实际文献需通过PubMed、Web of Science等平台以关键词"TSSK3 recombinant protein"或"TSSK3 kinase"检索。若需具体文献，建议补充研究背景或联系学术数据库查询。
**Background of TSSK3 Recombinant Protein**
TSSK3 (testis-specific serine/threonine kinase 3) is a member of the testis-specific serine/threonine kinase family, which is predominantly expressed in the male germline during late spermatogenesis. This kinase family plays critical roles in spermiogenesis, particularly in sperm maturation, chromatin condensation, and acrosome formation. TSSK3. encoded by the *TSSK3* gene in humans, is characterized by its testis-specific expression pattern and its conserved kinase domain, which regulates substrate phosphorylation during sperm development.
The recombinant TSSK3 protein is engineered using molecular cloning techniques, often expressed in *E. coli* or mammalian cell systems to ensure proper folding and post-translational modifications. Its recombinant form enables detailed biochemical studies, including kinase activity assays, substrate identification, and interaction mapping. Research has highlighted TSSK3's involvement in signaling pathways essential for sperm morphogenesis and fertility. Knockout mouse models deficient in TSSK3 exhibit male sterility due to impaired sperm maturation, underscoring its non-redundant role in reproduction.
Beyond basic research, TSSK3 recombinant protein is pivotal in drug discovery, particularly for male contraceptives or infertility treatments. Its selective expression in testes minimizes off-target effects, making it an attractive therapeutic target. Additionally, aberrant TSSK3 expression has been explored in germ cell tumors, linking its activity to oncogenic pathways.
Overall, TSSK3 recombinant protein serves as a vital tool for deciphering molecular mechanisms in spermatogenesis and developing targeted reproductive health interventions.
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