| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRDM7 |
| Uniprot No | Q9NQW5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-492aa |
| 氨基酸序列 | MSPERSQEESPEGDTERTERKPMVKDAFKDISIYFTKEEWAEMGDWEKTRYRNVKMNYNALITVGLRATRPAFMCHRRQAIKLQVDDTEDSDEEWTPRQQVKPPWMAFRGEQSKHQKGMPKASFNNESSLRELSGTPNLLNTSDSEQAQKPVSPPGEASTSGQHSRLKLELRRKETEGKMYSLRERKGHAYKEISEPQDDDYLYCEMCQNFFIDSCAAHGPPTFVKDSAVDKGHPNRSALSLPPGLRIGPSGIPQAGLGVWNEASDLPLGLHFGPYEGRITEDEEAANSGYSWLITKGRNCYEYVDGKDKSSANWMRYVNCARDDEEQNLVAFQYHRQIFYRTCRVIRPGCELLVWSGDEYGQELGIRSSIEPAESLGQAVNCWSGMGMSMARNWASSGAASGRKSSWQGENQSQRSIHVPHAVWPFQVKNFSVNMWNAITPLRTSQDHLQENFSNQRIPAQGIRIRSGNILIHAAVMTKPKVKRSKKGPNS |
| 预测分子量 | 59.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为示例性参考文献(具体文献需通过学术数据库核实)：
1. **《PRDM7 encodes a histone methyltransferase critical for human spermatogenesis》**
- 作者：Wang, Y. et al.
- 摘要：研究揭示了PRDM7作为组蛋白甲基转移酶的功能，通过重组蛋白实验证明其在精子发生中调控关键基因的表观遗传修饰。
2. **《Structural and functional analysis of the PRDM7 zinc finger domain》**
- 作者：Smith, J.R. & Lee, K.
- 摘要：利用重组PRDM7蛋白进行结构解析，发现其锌指结构域特异性地结合DNA靶序列，并参与胚胎发育的转录调控。
3. **《PRDM7重组蛋白在乳腺癌细胞中的异常表达及功能》**
- 作者：Zhang, L. et al.
- 摘要：通过重组PRDM7蛋白体外实验，发现其表达下调可抑制乳腺癌细胞增殖，可能与表观遗传调控通路相关。
4. **《Recombinant PRDM7 protein exhibits DNA-binding and chromatin remodeling activities in vitro》**
- 作者：Garcia, M. et al.
- 摘要：体外实验表明重组PRDM7蛋白可通过甲基转移酶活性和染色质重塑参与基因组稳定性维持。
**备注**：PRDM7研究相对较少，以上内容为领域内可能方向示例，建议通过PubMed或Google Scholar以“PRDM7 recombinant”为关键词检索最新文献。
PRDM7 (PR/SET Domain 7) is a member of the PRDM protein family characterized by an N-terminal PR/SET domain, a structural motif homologous to the catalytic SET domain involved in histone methyltransferase activity. While the functional roles of PRDM7 remain less characterized compared to other PRDM members (e.g., PRDM9 in meiosis), emerging evidence suggests its involvement in epigenetic regulation. The protein typically contains zinc finger arrays, which mediate sequence-specific DNA binding or protein-protein interactions. Phylogenetically, PRDM7 appears conserved in mammals but exhibits unique features, including potential post-translational modifications that modulate its activity.
Recombinant PRDM7 is engineered for in vitro studies to overcome challenges in isolating endogenous protein due to low expression levels. Produced via heterologous expression systems (e.g., E. coli, mammalian cells), recombinant PRDM7 enables biochemical characterization of its methyltransferase activity, substrate specificity, and interaction partners. Tagging strategies (e.g., His-tag, GST-tag) facilitate purification via affinity chromatography. Recent studies utilize recombinant PRDM7 to explore its role in chromatin remodeling, particularly in embryonic development and germline reprogramming. Intriguingly, PRDM7 may act as a histone H3K4 trimethyltransferase, though conflicting reports highlight context-dependent enzymatic activity. Structural analyses of recombinant protein suggest conformational flexibility influencing its regulatory functions. Dysregulation of PRDM7 has been tentatively linked to genomic instability and certain cancers, though mechanistic insights remain sparse. Current research prioritizes resolving its enzymatic specificity and genome-wide binding patterns using recombinant protein-based assays.
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