| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | WRKY2 |
| Uniprot No | Q9FG77 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 175-405aa |
| 氨基酸序列 | FHPVSRSSSSFFQGTTEMMSVDYGNYNNRSSSHQSAEEVKPGSENIESSNLYGIETDNQNGQNKTSDVTTNTSLETVDHQEEEEEQRRGDSMAGGAPAEDGYNWRKYGQKLVKGSEYPRSYYKCTNPNCQVKKKVERSREGHITEIIYKGAHNHLKPPPNRRSGMQVDGTEQVEQQQQQRDSAATWVSCNNTQQQGGSNENNVEEGSTRFEYGNQSGSIQAQTGGQYESGD |
| 预测分子量 | 27.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于 **WRKY2重组蛋白** 的模拟参考文献(内容为示例，非真实文献)：
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1. **文献名称**: *"Functional characterization of recombinant WRKY2 transcription factor in Arabidopsis stress responses"*
**作者**: Liu Y, Zhang H, Chen L.
**摘要**: 本研究通过原核表达系统纯化拟南芥WRKY2重组蛋白，发现其在体外特异性结合干旱胁迫相关基因启动子的W-box元件。实验表明WRKY2可能通过调控ABA信号通路增强植物耐旱性。
2. **文献名称**: *"Structural analysis of WRKY2 DNA-binding domain using recombinant protein expression"*
**作者**: Kim S, Park JH, Lee M.
**摘要**: 作者解析了重组WRKY2蛋白的DNA结合结构域晶体结构，揭示其锌指模体与DNA相互作用的分子机制，为WRKY家族蛋白的功能进化提供结构依据。
3. **文献名称**: *"Recombinant WRKY2 enhances salt tolerance in rice by regulating ion homeostasis genes"*
**作者**: Wang X, Li R, Zhao Q.
**摘要**: 通过在大肠杆菌中表达水稻WRKY2重组蛋白，发现其能够激活SOS1和NHX1等离子转运基因的启动子活性，暗示WRKY2在植物耐盐性中的调控作用。
4. **文献名称**: *"Post-translational modification of recombinant WRKY2 modulates its transcriptional activity"*
**作者**: Gupta A, Singh PK.
**摘要**: 研究利用昆虫细胞系统表达WRKY2重组蛋白，发现其磷酸化和泛素化修饰可动态调控其与靶基因的结合能力，影响植物免疫应答效率。
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如需真实文献，建议在 **PubMed** 或 **Web of Science** 中检索关键词：`WRKY2 recombinant protein` + `expression`/`function`/`structure`。
**Background of WRKY2 Recombinant Protein**
The WRKY family of transcription factors is a large group of plant-specific regulatory proteins characterized by the presence of a conserved WRKY domain, which binds to the W-box DNA motif [(T/G)TGAC(C/T)] to modulate gene expression. These proteins play pivotal roles in plant growth, development, and responses to biotic/abiotic stresses. WRKY2. a member of this family, has been identified as a key regulator in specific signaling pathways, particularly in *Arabidopsis thaliana*. Studies suggest its involvement in seed development, germination, and responses to environmental cues such as drought, salinity, and pathogen attacks.
The recombinant WRKY2 protein is engineered to enable detailed biochemical and functional analyses. Typically produced via heterologous expression systems (e.g., *E. coli* or insect cells*), the recombinant protein retains the DNA-binding activity of native WRKY2. allowing researchers to study its interaction with target promoters, post-translational modifications, and regulatory networks. Its production often involves fusion tags (e.g., His-tag) for simplified purification and detection.
Research on WRKY2 recombinant protein has advanced understanding of its molecular mechanisms, including its role in ABA (abscisic acid) signaling during seed dormancy and stress adaptation. Additionally, structural studies using recombinant WRKY2 have elucidated how its WRKY domain recognizes DNA motifs, providing insights into its specificity and regulatory versatility.
The application of recombinant WRKY2 extends to biotechnological innovations, such as developing stress-tolerant crops through genetic engineering. By manipulating WRKY2 expression or activity, scientists aim to enhance plant resilience, highlighting its agricultural relevance. Overall, WRKY2 recombinant protein serves as a critical tool for dissecting plant stress responses and engineering improved crop traits.
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