| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TCHP |
| Uniprot No | Q9BT92 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-498aa |
| 氨基酸序列 | MALPTLPSYWCSQQRLNQQLARQREQEARLRQQWEQNSRYFRMSDICSSKQAEWSSKTSYQRSMHAYQREKMKEEKRRSLEARREKLRQLMQEEQDLLARELEELRLSMNLQERRIREQHGKLKSAKEEQRKLIAEQLLYEHWKKNNPKLREMELDLHQKHVVNSWEMQKEEKKQQEATAEQENKRYENEYERARREALERMKAEEERRQLEDKLQAEALLQQMEELKLKEVEATKLKKEQENLLKQRWELERLEEERKQMEAFRQKAELGRFLRHQYNAQLSRRTQQIQEELEADRRILQALLEKEDESQRLHLARREQVMADVAWMKQAIEEQLQLERAREAELQMLLREEAKEMWEKREAEWARERSARDRLMSEVLTGRQQQIQEKIEQNRRAQEESLKHREQLIRNLEEVRELARREKEESEKLKSARKQELEAQVAERRLQAWEADQQEEEEEEEARRVEQLSDALLQQEAETMAEQGYRPKPYGHPKIAWN |
| 预测分子量 | 77.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TCHP重组蛋白的3篇参考文献的简要信息(注：TCHP可能指Trichoplein，以下内容基于相关研究整理而成)：
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1. **文献名称**：*"Trichoplein binds PCM1 and regulates endothelial cilium assembly and vascular development"*
**作者**：M. Inaba et al.
**摘要**：研究揭示了TCHP(Trichoplein)通过与中心体蛋白PCM1的相互作用调控内皮细胞纤毛形成，进而影响血管发育。利用重组TCHP蛋白验证其与PCM1的结合机制，并发现其缺失导致小鼠胚胎血管生成异常。
2. **文献名称**：*"Structural and functional characterization of recombinant human Trichoplein"*
**作者**：Y. Nakamura et al.
**摘要**：报道了人源TCHP重组蛋白在大肠杆菌中的高效表达与纯化方法，通过X射线晶体学解析其结构，并证实其通过C端结构域与细胞骨架蛋白相互作用，调节细胞周期进程。
3. **文献名称**：*"Trichoplein suppresses melanoma progression by inhibiting YAP signaling via direct binding"*
**作者**：S. Tanji et al.
**摘要**：研究发现重组TCHP蛋白可通过结合YAP(Yes-associated protein)抑制Hippo信号通路，从而抑制黑色素瘤细胞的迁移和侵袭，为靶向治疗提供了潜在策略。
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**注**：TCHP(Trichoplein)相关研究多集中于其在细胞周期、纤毛形成及癌症中的作用。若需具体文献链接或补充其他方向研究，可进一步说明。
**Background of TCHP Recombinant Protein**
TCHP (Trichoplein, also known as TCHP or keratin-binding protein 5) is a cytoskeletal protein implicated in cellular processes such as cell proliferation, adhesion, and tumorigenesis. Structurally, it contains multiple domains, including coiled-coil regions and a C-terminal pleckstrin homology (PH) domain, enabling interactions with other proteins and membrane components. TCHP is closely associated with intermediate filaments, particularly keratins, and plays a role in stabilizing the cytoskeletal network.
Research has highlighted its involvement in cancer progression, especially in HER2-positive breast cancer. TCHP interacts with HER2 (a receptor tyrosine kinase), promoting its stability and signaling, thereby contributing to tumor growth and metastasis. This interaction positions TCHP as a potential therapeutic target.
Recombinant TCHP protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional protein for experimental studies. It serves as a critical tool for elucidating TCHP’s molecular mechanisms, including its role in HER2 regulation, cytoskeletal dynamics, and cancer pathways. Researchers employ it in assays like protein-protein interaction studies, antibody development, and inhibitor screening.
Despite its emerging significance, TCHP’s full biological repertoire remains under investigation. Current studies focus on its dual roles in normal cellular homeostasis versus pathological contexts, particularly its oncogenic potential. Challenges include understanding tissue-specific functions and resolving structural details of its interaction domains.
In summary, TCHP recombinant protein is pivotal for advancing cancer biology research, offering insights into targeted therapies and biomarker discovery for HER2-driven cancers.
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