| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SNTG1 |
| Uniprot No | Q9NSN8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-517aa |
| 氨基酸序列 | MDFRTACEETKTGICLLQDGNQEPFKVRLHLAKDILMIQEQDVICVSGEPFYSGERTVTIRRQTVGGFGLSIKGGAEHNIPVVVSKISKEQRAELSGLLFIGDAILQINGINVRKCRHEEVVQVLRNAGEEVTLTVSFLKRAPAFLKLPLNEDCACAPSDQSSGTSSPLCDSGLHLNYHPNNTDTLSCSSWPTSPGLRWEKRWCDLRLIPLLHSRFSQYVPGTDLSRQNAFQVIAVDGVCTGIIQCLSAEDCVDWLQAIATNISNLTKHNIKKINRNFPVNQQIVYMGWCEAREQDPLQDRVYSPTFLALRGSCLYKFLAPPVTTWDWTRAEKTFSVYEIMCKILKDSDLLDRRKQCFTVQSESGEDLYFSVELESDLAQWERAFQTATFLEVERIQCKTYACVLESHLMGLTIDFSTGFICFDAATKAVLWRYKFSQLKGSSDDGKSKIKFLFQNPDTKQIEAKELEFSNLFAVLHCIHSFFAAKVACLDPLFLGNQATASTAASSATTSKAKYTT |
| 预测分子量 | 63.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SNTG1重组蛋白的3篇模拟参考文献(非真实文献，仅供示例参考)：
1. **《重组人SNTG1蛋白的表达及功能研究》**
作者：李明等
摘要：通过大肠杆菌表达系统成功获得高纯度SNTG1重组蛋白，并证明其与β-dystroglycan在细胞膜上的相互作用，提示其在肌肉细胞信号转导中的潜在作用。
2. **《SNTG1重组蛋白调控神经元突触可塑性的机制》**
作者：Chen L, et al.
摘要：利用体外纯化的SNTG1重组蛋白发现，其通过结合突触后支架蛋白PSD-95.影响AMPA受体定位，从而调节海马神经元的突触传递效率。
3. **《SNTG1重组蛋白在乳腺癌细胞迁移中的作用》**
作者：Wang Y, et al.
摘要：研究发现SNTG1重组蛋白可抑制乳腺癌细胞侵袭，其机制可能与下调ERK/MAPK通路活性及减少基质金属蛋白酶(MMPs)分泌相关。
注：以上内容为假设性文献，实际研究中请通过PubMed或Google Scholar检索真实文献。
SNTG1 (Syntrophin Gamma 1) is a member of the syntrophin family of cytoplasmic scaffold proteins, which play critical roles in organizing membrane-associated signaling complexes. These proteins are characterized by conserved domain structures, including PDZ domains for protein-protein interactions, PH (pleckstrin homology) domains for membrane localization, and SU (syntrophin unique) domains. SNTG1 is widely expressed in tissues, with notable presence in the nervous system, skeletal muscle, and heart, where it interacts with dystrophin-associated protein complexes (DAPC) to maintain structural integrity and facilitate signal transduction.
Recombinant SNTG1 protein is engineered to study its molecular interactions, particularly in neurological and muscular disorders. Syntrophins, including SNTG1. are implicated in anchoring ion channels, receptors, and kinases to the cytoskeleton. For example, SNTG1 binds to voltage-gated sodium channels (Nav1.2/1.6) in neurons and regulates their clustering at nodes of Ranvier. Dysregulation of SNTG1 has been linked to neurodevelopmental defects and muscular dystrophy pathologies, underscoring its role in cellular stability and excitability.
The production of recombinant SNTG1 typically involves cloning its cDNA into expression vectors (e.g., bacterial, insect, or mammalian systems) to yield purified, functional protein. This enables in vitro studies on its binding partners, post-translational modifications, and disease-associated mutations. Researchers utilize SNTG1 recombinant protein to explore mechanisms underlying conditions like epilepsy, autism spectrum disorders, and cardiomyopathy, as well as to screen potential therapeutic compounds targeting syntrophin-mediated pathways. Its applications extend to structural biology, antibody development, and functional assays probing membrane-cytoskeleton crosstalk.
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