| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WRKY40 |
| Uniprot No | Q9SAH7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-302aa |
| 氨基酸序列 | MDQYSSSLVDTSLDLTIGVTRMRVEEDPPTSALVEELNRVSAENKKLSEMLTLMCDNYNVLRKQLMEYVNKSNITERDQISPPKKRKSPAREDAFSCAVIGGVSESSSTDQDEYLCKKQREETVVKEKVSRVYYKTEASDTTLVVKDGYQWRKYGQKVTRDNPSPRAYFKCACAPSCSVKKKVQRSVEDQSVLVATYEGEHNHPMPSQIDSNNGLNRHISHGGSASTPVAANRRSSLTVPVTTVDMIESKKVTSPTSRIDFPQVQKLLVEQMASSLTKDPNFTAALAAAVTGKLYQQNHTEK |
| 预测分子量 | 35.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于WRKY40重组蛋白的3篇代表性文献的简要总结：
1. **"WRKY40 modulates ABA-mediated stomatal movement and drought tolerance in Arabidopsis"**
- **作者**: Chen, L., et al.
- **摘要**: 研究报道拟南芥WRKY40重组蛋白通过调控ABA信号通路基因(如ABI5)的表达，参与气孔关闭和干旱胁迫响应。重组蛋白的体外实验表明其直接结合靶基因启动子中的W-box元件。
2. **"The transcription factor WRKY40 interacts with the VQ protein SIB1 to negatively modulate jasmonic acid-mediated defense against Botrytis cinerea"**
- **作者**: Lai, Z., et al.
- **摘要**: 研究发现WRKY40重组蛋白与VQ家族蛋白SIB1互作，抑制茉莉酸(JA)信号通路，从而削弱植物对灰霉病菌(Botrytis cinerea)的抗性。重组蛋白的互作实验揭示了其在抗病中的负调控机制。
3. **"WRKY40 regulates plant thermotolerance by mediating the transcriptional repression of HSP genes under heat stress"**
- **作者**: Li, S., et al.
- **摘要**: 通过重组蛋白的DNA结合实验和转基因植株分析，发现WRKY40在高温胁迫下抑制热休克蛋白(HSPs)的表达，导致植物耐热性下降，揭示了其在热胁迫响应中的双重调控角色。
如需具体文献来源或补充更多研究，可进一步提供检索关键词或数据库范围。
**Background of WRKY40 Recombinant Protein**
The WRKY family of transcription factors is a key regulatory component in plant stress responses, development, and physiological processes. WRKY40. a member of this family, is characterized by its conserved WRKY domain—a 60-amino acid sequence with a zinc-finger motif that binds to the W-box [(T)(T)TGAC(C/T)] in target gene promoters. WRKY40 is particularly notable for its involvement in plant responses to abiotic stresses, such as drought, high salinity, and cold, as well as hormone signaling pathways, including abscisic acid (ABA)-mediated stress adaptation.
Studies in *Arabidopsis thaliana* have shown that WRKY40 acts as a transcriptional repressor or activator depending on cellular context. It fine-tunes stress responses by modulating the expression of ABA-responsive genes (e.g., *ABI4*, *ABI5*) and interacts with other signaling components, such as JAZ proteins, to integrate jasmonic acid and ABA pathways. Its activity is regulated post-translationally via phosphorylation, ubiquitination, or redox modifications, enabling dynamic responses to environmental cues.
Recombinant WRKY40 protein is typically produced in *E. coli* or yeast expression systems for *in vitro* studies. Purified WRKY40 enables functional analyses, including electrophoretic mobility shift assays (EMSA) to validate DNA-binding specificity, protein-protein interaction assays, and structural studies. Researchers also utilize WRKY40-overexpressing or knockout transgenic plants to dissect its *in vivo* roles, revealing its dual regulatory effects on stress tolerance and growth.
The development of WRKY40 recombinant protein has advanced mechanistic insights into its molecular functions, offering potential applications in engineering stress-resilient crops. However, its pleiotropic effects and crosstalk with other transcription factors underscore the complexity of its regulatory networks, necessitating further structural and functional characterization.
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