| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CXXC4 |
| Uniprot No | Q9H2H0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-198aa |
| 氨基酸序列 | MHHRNDSQRLGKAGCPPEPSLQMANTNFLSTLSPEHCRPLAGECMNKLKCGAAEAEIMNLPERVGTFSAIPALGGISLPPGVIVMTALHSPAAASAAVTDSAFQIANLADCPQNHSSSSSSSSGGAGGANPAKKKRKRCGVCVPCKRLINCGVCSSCRNRKTGHQICKFRKCEELKKKPGTSLERTPVPSAEAFRWFF |
| 预测分子量 | 48.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CXXC4重组蛋白的3篇文献摘要信息(虚构示例，仅供格式参考)：
1. **文献名称**: *CXXC4 modulates Wnt/β-catenin signaling by binding to Dishevelled*
**作者**: Li Y, et al.
**摘要**: 本研究通过体外重组CXXC4蛋白实验，发现其直接与Dishevelled蛋白相互作用，抑制Wnt/β-catenin信号通路活性，可能为结直肠癌治疗提供新靶点。
2. **文献名称**: *Recombinant CXXC4 suppresses myeloid neoplasms via TET2 activation*
**作者**: Wang X, et al.
**摘要**: 利用重组CXXC4蛋白处理骨髓细胞，证实其通过增强TET2介导的DNA去甲基化，抑制骨髓增生异常综合征(MDS)模型中的异常造血分化。
3. **文献名称**: *Structural analysis of CXXC4 in iron metabolism regulation*
**作者**: Tanaka K, et al.
**摘要**: 通过晶体结构解析发现，重组CXXC4蛋白的锌指结构域可螯合铁离子，提示其在细胞铁稳态调控中的潜在作用，与贫血病理机制相关。
(注：以上内容为模拟生成，实际文献需通过PubMed/Google Scholar检索关键词“CXXC4 recombinant protein”或“CXXC4 function”获取。)
**Background of CXXC4 Recombinant Protein**
CXXC4. also known as Idax (Inhibitor of Dishevelled and Axin), is a nuclear protein encoded by the *CXXC4* gene. It belongs to the CXXC zinc finger domain-containing protein family, which is characterized by a conserved CXXC motif involved in binding unmethylated CpG DNA regions. This domain enables CXXC4 to participate in epigenetic regulation, particularly in modulating Wnt/β-catenin signaling, a critical pathway for cell proliferation, differentiation, and apoptosis.
CXXC4 acts as a negative regulator of the Wnt pathway by interacting with Dishevelled (Dvl), a key mediator of Wnt signal transduction. Through this interaction, CXXC4 promotes the degradation of Dvl proteins, thereby suppressing β-catenin stabilization and downstream transcriptional activation. Dysregulation of CXXC4 has been implicated in various diseases, including cancers (e.g., colorectal, hepatocellular carcinoma) and inflammatory disorders, where aberrant Wnt signaling often plays a pathogenic role.
Recombinant CXXC4 protein is engineered using expression systems (e.g., *E. coli*, mammalian cells) to produce purified, functional protein for research applications. It typically retains the CXXC domain and Dvl-binding regions, enabling studies on Wnt pathway modulation, protein-protein interactions, and epigenetic mechanisms. Researchers utilize this tool to investigate CXXC4’s tumor-suppressive functions, its role in DNA methylation dynamics, and its potential as a therapeutic target. Additionally, recombinant CXXC4 aids in screening small molecules or biologics that could restore Wnt pathway homeostasis in disease contexts.
Overall, CXXC4 recombinant protein serves as a vital resource for dissecting molecular pathways linked to development and disease, offering insights into novel therapeutic strategies.
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