| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SP140L |
| Uniprot No | Q9H930 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-580aa |
| 氨基酸序列 | MAGGGSDLSTRGLNGGVSQVANEMNHLPAHSQSLQRLFTEDQDVDEGLVYDTVFKHFKRHKLEISNAIKKTFPFLEGLRDRELITNKMFEDSEDSCRNLVPVQRVVYNVLSELEKTFNLSVLEALFSEVNMQEYPDLIHIYKSFKNAIQDKLSFQESDRKEREERPDIKLSLKQGEVPESPEARKESDQACGKMDTVDIANNSTLGKPKRKRRKKKGHGWSRMGTRTQKNNQQNDNSKADGQLVSSEKKANMNLKDLSKIRGRKRGKPGTHFTQSDRAPQKRVRSRASRKHKDETVDFQAPLLPVTCGGVKGILHKEKLEQGTLAKCIQTEDGKWFTPMEFEIKGGYARSKNWRLSVRCGGWPLRRLMEEGSLPNPPRIYYRNKKRILKSQNNSSVDPCMRNLDECEVCRDGGELFCCDTCSRVFHEDCHIPPVESEKTPWNCIFCRMKESPGSQQCCQESEVLERQMCPEEQLKCEFLLLKVYCCSESSFFAKIPYYYYIREACQGLKEPMWLDKIKKRLNEHGYPQVEGFVQDMRLIFQNHRASYKYKDFGQMGLRLEAEFEKDFKEVFAIQETNGNS |
| 预测分子量 | 70.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SP140L重组蛋白的3篇代表性文献信息(注：以下内容为模拟示例，实际文献可能需要通过学术数据库检索获取)：
1. **文献名称**：《SP140L重组蛋白的体外表达及其免疫调节功能研究》
**作者**：Zhang L, Wang Y, et al.
**摘要**：研究成功在大肠杆菌中表达并纯化了SP140L重组蛋白，发现其能够显著抑制巨噬细胞中促炎细胞因子的释放，提示其在免疫调控中的潜在作用。
2. **文献名称**：《SP140L蛋白结构解析及其与染色质相互作用机制》
**作者**：Smith J, Brown K, et al.
**摘要**：通过X射线晶体学解析了SP140L重组蛋白的N端结构域，揭示了其通过PHD结构域特异性识别组蛋白H3K4me2修饰，进而参与表观遗传调控。
3. **文献名称**：《SP140L重组蛋白在慢性淋巴细胞白血病中的异常表达及临床意义》
**作者**：Chen X, Liu R, et al.
**摘要**：研究发现SP140L在慢性淋巴细胞白血病(CLL)患者中表达显著下调，体外实验表明重组SP140L可抑制白血病细胞增殖，提示其作为治疗靶点的可能性。
**提示**：实际文献需通过PubMed、Web of Science等平台以“SP140L recombinant protein”、“SP140L function”等关键词检索。若研究较少，可扩展至SP140相关文献。
**Background of SP140L Recombinant Protein**
SP140L (Speckled Protein 140. Lymphocyte-specific isoform) is a member of the SP100/SP140 family of nuclear body proteins, which are implicated in transcriptional regulation, chromatin remodeling, and innate immune responses. Structurally, SP140L contains multiple functional domains, including a SP100/SP140 nuclear localization sequence, a SAND domain (involved in DNA binding), and a PHD (plant homeodomain) finger, which mediates protein-protein interactions and chromatin recognition. This protein is predominantly expressed in immune cells, such as lymphocytes, and localizes to nuclear speckles or PML (promyelocytic leukemia) bodies, dynamic subnuclear compartments associated with gene regulation and antiviral defense.
SP140L has been linked to immune-related pathways, particularly interferon signaling, where it may act as a transcriptional modulator. Studies suggest it plays a role in restricting viral replication by promoting chromatin condensation at viral integration sites, thereby inhibiting pathogen proliferation. Dysregulation of SP140L has been observed in autoimmune diseases (e.g., multiple sclerosis, Crohn’s disease) and hematological malignancies, where altered expression correlates with disease progression or therapeutic resistance.
Recombinant SP140L protein, produced via expression systems like *E. coli* or mammalian cells, is utilized to study its biochemical properties, interactions, and functional mechanisms. It serves as a tool for investigating epigenetic regulation, immune cell signaling, and host-pathogen interactions. Additionally, SP140L is explored as a potential therapeutic target or biomarker in autoimmune disorders and cancers, given its disease-specific expression patterns. Recent research also highlights its involvement in maintaining genomic stability, further underscoring its relevance in both basic and translational biomedical studies.
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