| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C8orf4 |
| Uniprot No | Q9NR00 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-106aa |
| 氨基酸序列 | MKAKRSHQAVIMSTSLRVSPSIHGYHFDTASRKKAVGNIFENTDQESLERLFRNSGDKKAEERAKIIFAIDQDVEEKTRALMALKKRTKDKLFQFLKLRKYSIKVH |
| 预测分子量 | 39.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于C8orf4重组蛋白的示例参考文献(请注意，以下内容为模拟示例，实际文献需通过学术数据库查询)：
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1. **文献名称**: *TC1 (C8orf4) enhances Wnt/β-catenin signaling by stabilizing β-catenin in hepatocellular carcinoma*
**作者**: Li, Y., Wang, X., & Zhang, M.
**摘要**: 本研究通过重组TC1蛋白实验，发现其通过与β-catenin直接结合，抑制其降解，从而增强Wnt信号通路活性，促进肝癌细胞增殖和侵袭。
2. **文献名称**: *Recombinant C8orf4 protein promotes angiogenesis via PI3K/AKT pathway activation*
**作者**: Chen, L., et al.
**摘要**: 利用重组C8orf4蛋白处理内皮细胞，发现其通过激活PI3K/AKT通路，上调促血管生成因子(如VEGF)，显著促进体外血管形成能力。
3. **文献名称**: *Expression and functional characterization of recombinant C8orf4 in colorectal cancer models*
**作者**: Kim, S., et al.
**摘要**: 报道了在大肠杆菌中高效表达并纯化重组C8orf4蛋白的方法，并证明其在结直肠癌模型中通过激活NF-κB通路抑制细胞凋亡，促进肿瘤生长。
4. **文献名称**: *C8orf4 recombinant protein exacerbates metabolic dysfunction in obesity-associated insulin resistance*
**作者**: Gupta, R., et al.
**摘要**: 动物实验显示，注射重组C8orf4蛋白会加剧肥胖小鼠的胰岛素抵抗，其机制可能与干扰IRS-1信号通路及诱导慢性炎症相关。
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**提示**：以上文献为示例，实际研究中请通过PubMed、Google Scholar等平台以关键词“C8orf4 recombinant protein”或“TC1 protein function”检索最新文献，并核实作者与期刊信息。
C8orf4 (Chromosome 8 Open Reading Frame 4), also known as THAP6 or TARSS, is a gene located on human chromosome 8p21.3. The encoded protein is evolutionarily conserved and ubiquitously expressed in tissues, with higher levels observed in the brain, heart, and reproductive organs. Structurally, C8orf4 contains a THAP domain—a zinc-coordinating motif involved in DNA binding and protein interactions—suggesting potential roles in transcriptional regulation. However, its precise molecular functions remain incompletely characterized.
Research indicates C8orf4 may participate in cellular processes such as apoptosis, proliferation, and differentiation. It interacts with components of the NF-κB signaling pathway and modulates p53 activity, linking it to stress responses and tumorigenesis. Notably, C8orf4 is downregulated in several cancers (e.g., gastric, colorectal), where it may act as a tumor suppressor by inhibiting cell migration and invasion. Paradoxically, overexpression studies in glioblastoma show pro-survival effects, highlighting context-dependent roles.
Recombinant C8orf4 protein is typically produced via bacterial or mammalian expression systems, enabling functional studies. Tagged versions (e.g., His, GST) facilitate purification and interaction assays. Current applications include antibody generation, protein-DNA/RNA interaction analyses, and mechanistic studies in cancer models. Despite progress, unresolved questions persist regarding its physiological targets, regulation, and therapeutic potential. Further investigation of C8orf4 could clarify its dual roles in cancer and inform strategies for targeted therapies.
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