| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPS19BP1 |
| Uniprot No | Q86WX3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-145aa |
| 氨基酸序列 | MSAALLRRGLELLAASEAPRDPPGQAKPRGAPVKRPRKTKAIQAQKLRNSAKGKVPKSALDEYRKRECRDHLRVNLKFLTRTRSTVAESVSQQILRQNRGRKACDRPVAKTKKKKAEGTVFTEEDFQKFQQEYFGS |
| 预测分子量 | 42.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RPS19BP1重组蛋白的3篇参考文献的示例(注：以下内容为模拟生成，实际文献可能需要进一步检索验证)：
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1. **文献名称**：*"Cloning, Expression, and Functional Analysis of Recombinant RPS19BP1 in Ribosome Biogenesis"*
**作者**：Zhang Y, et al.
**摘要**：本研究成功克隆了人源RPS19BP1基因，并利用大肠杆菌系统表达纯化了重组蛋白。实验表明，RPS19BP1可能通过与核糖体蛋白S19(RPS19)相互作用，调控核糖体RNA的加工过程，提示其在核糖体生物合成中的潜在功能。
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2. **文献名称**：*"RPS19BP1 Recombinant Protein Suppresses Tumor Growth via Modulating p53 Signaling Pathway"*
**作者**：Lee J, Kim S.
**摘要**：通过体外表达RPS19BP1重组蛋白，作者发现其能够增强p53的稳定性并激活下游抑癌基因，从而抑制多种癌细胞系的增殖。该研究为RPS19BP1在癌症治疗中的应用提供了实验依据。
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3. **文献名称**：*"Structural Characterization of RPS19BP1 and Its Interaction with Ribosomal Subunits"*
**作者**：Smith R, et al.
**摘要**：利用X射线晶体学解析了RPS19BP1重组蛋白的三维结构，并发现其通过特定结构域与核糖体小亚基结合。研究揭示了RPS19BP1在核糖体组装中的分子机制，为遗传性血液疾病的致病机制提供了新见解。
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如需获取真实文献，建议通过PubMed、Web of Science等数据库检索关键词“RPS19BP1 recombinant protein”或联系相关领域研究人员。
**Background of RPS19BP1 Recombinant Protein**
RPS19BP1 (Ribosomal Protein S19 Binding Protein 1) is a relatively understudied protein implicated in ribosome biogenesis and cellular homeostasis. It was initially identified through its interaction with ribosomal protein S19 (RPS19), a component of the 40S ribosomal subunit. RPS19 itself is critical for ribosome assembly, and mutations in its gene are linked to Diamond-Blackfan anemia (DBA), a rare congenital bone marrow failure syndrome. RPS19BP1 is hypothesized to act as a regulatory partner or chaperone for RPS19. potentially modulating its stability, localization, or function during ribosome synthesis.
The recombinant form of RPS19BP1 is engineered for experimental studies, enabling researchers to explore its biochemical properties and interactions. Recombinant RPS19BP1 is typically produced in heterologous expression systems (e.g., *E. coli* or mammalian cells*) using DNA sequences cloned into expression vectors, often fused with tags like His-tag or GFP for purification and detection. Its production facilitates investigations into its role in ribosome assembly, nucleolar stress responses, and potential involvement in diseases like DBA or cancers where ribosome dysfunction is observed.
Emerging studies suggest RPS19BP1 may also intersect with pathways beyond ribosome biogenesis, including p53-mediated stress signaling, given the nucleolus’s role as a stress sensor. However, mechanistic details remain sparse, underscoring the need for further research. Recombinant RPS19BP1 serves as a vital tool to dissect these pathways, offering insights into cellular proliferation, apoptosis, and disease mechanisms linked to ribosomal defects.
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