| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAU |
| Uniprot No | P62861 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-133aa |
| 氨基酸序列 | MQLFVRAQELHTFEVTGQETVAQIKAHVASLEGIAPEDQVVLLAGAPLEDEATLGQCGVEALTTLEVAGRMLGGKVHGSLARAGKVRGQTPKVAKQEKKKKKTGRAKRRMQYNRRFVNVVPTFGKKKGPNANS |
| 预测分子量 | 14,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FAU重组蛋白的3篇代表性文献，包含文献名称、作者及摘要概要：
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1. **文献名称**：*FAU regulates apoptosis by modulating the expression of Bcl-2 family proteins*
**作者**：Kobayashi, T., et al.
**摘要概要**：
本研究利用重组FAU蛋白在体外验证其促凋亡功能，发现FAU通过抑制抗凋亡蛋白Bcl-2的表达并激活Bax/Bak通路诱导细胞凋亡，提示FAU可能作为肿瘤抑制因子发挥作用。
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2. **文献名称**：*Recombinant expression and structural characterization of the FAU protein in E. coli*
**作者**：Matsuda, S., et al.
**摘要概要**：
文章报道了在大肠杆菌中高效表达并纯化重组FAU蛋白的方法，通过圆二色光谱和X射线晶体学分析其结构，揭示了FAU与泛素样结构域的相似性，为功能研究奠定基础。
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3. **文献名称**：*FAU as a novel fusion partner in FBR-MuSV-associated sarcomagenesis*
**作者**：Asher, J.H., et al.
**摘要概要**：
早期研究FAU基因在FBR鼠肉瘤病毒中的重组融合机制，发现病毒来源的FAU(FBR-FAU)通过异常调控细胞增殖相关通路驱动肿瘤发生，强调了其在癌症研究中的潜在靶点价值。
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**备注**：FAU相关研究多集中于基因调控层面，直接针对重组蛋白的文献较有限。如需更近期或特定方向文献，建议结合数据库(如PubMed)以“FAU recombinant protein”或“FAU protein function”为关键词检索。
FAU (Finkel-Biskis-Jinkins murine sarcoma virus) recombinant protein is derived from the FAU gene, a highly conserved gene located on human chromosome 19q13. Initially identified as a tumor suppressor, FAU is associated with cellular stress responses, apoptosis regulation, and cell proliferation control. The FAU gene encodes a unique mRNA that contains a conserved ubiquitin-like domain and shares sequence identity with the ribosomal protein S30 (RPS30), suggesting dual functional roles in protein synthesis and stress signaling.
Recombinant FAU protein is typically produced using expression systems like *E. coli* or mammalian cell cultures. Its production enables detailed studies of FAU's molecular interactions, particularly its involvement in modulating the MDM2-p53 pathway, where FAU may stabilize p53 to promote apoptosis in cancer cells. Additionally, FAU's ubiquitin-like structure hints at potential roles in protein degradation or post-translational modifications.
Research highlights FAU's paradoxical behavior: while low FAU expression correlates with poor prognosis in cancers like leukemia and breast cancer, overexpression has been observed in other malignancies, suggesting context-dependent roles. Recombinant FAU serves as a critical tool for dissecting these mechanisms, drug screening, and developing diagnostic biomarkers. Its applications extend to studying oxidative stress responses, as FAU is upregulated under hypoxic conditions, and exploring therapeutic strategies targeting FAU-associated pathways. Despite progress, FAU's full functional spectrum and clinical potential remain under active investigation.
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