| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MRPL9 |
| Uniprot No | Q9BYD2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 60-267aa |
| 氨基酸序列 | WKVPLAGEGRKPRLHRRHRVYKLVEDTKHRPKENLELILTQSVENVGVRGDLVSVKKSLGRNRLLPQGLAVYASPENKKLFEEEKLLRQEGKLEKIQTKAGEATVKFLKSCRLEVGMKNNVKWELNPEIVARHFFKNLGVVVAPHTLKLPEEPITRWGEYWCEVTVNGLDTVRVPMSVVNFEKPKTKRYKYWLAQQAAKAMAPTSPQI |
| 预测分子量 | 50.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MRPL9重组蛋白的模拟参考文献示例，供参考：
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1. **Structural Characterization of Recombinant MRPL9 and Its Role in Mitochondrial Ribosome Assembly**
*作者：Chen L, et al.*
摘要：该研究通过重组表达纯化人源MRPL9蛋白，结合X射线晶体学解析其三维结构，揭示了其在线粒体核糖体大亚基组装中的关键作用，为理解线粒体翻译机制提供结构基础。
2. **MRPL9 Overexpression Promotes Cancer Cell Proliferation via Mitochondrial Metabolism Reprogramming**
*作者：Wang Y, et al.*
摘要：研究发现MRPL9在结直肠癌中高表达，重组蛋白功能实验表明其通过增强线粒体翻译活性，促进癌细胞能量代谢和增殖，提示其作为潜在治疗靶点。
3. **Functional Analysis of MRPL9 Mutations in Neurodegenerative Disorders**
*作者：Smith J, et al.*
摘要：利用重组MRPL9突变体，研究揭示特定氨基酸变异导致线粒体蛋白合成缺陷，与帕金森病相关神经元退行性病变密切相关，为遗传机制提供新证据。
4. **Optimization of Recombinant MRPL9 Expression in E. coli for Biochemical Studies**
*作者：Kim H, et al.*
摘要：报道了一种高效重组表达MRPL9的大肠杆菌系统，通过密码子优化和纯化策略改进，获得高产量可溶性蛋白，适用于体外功能与相互作用研究。
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注：以上文献为模拟内容，实际研究中请通过学术数据库(如PubMed、Web of Science)检索真实文献。
**Background of MRPL9 Recombinant Protein**
MRPL9 (Mitochondrial Ribosomal Protein L9) is a core component of the mitochondrial ribosome large subunit, essential for mitochondrial protein synthesis. Mitochondria, the energy-producing organelles, possess their own translation machinery to synthesize subunits of the oxidative phosphorylation (OXPHOS) complexes, which are critical for ATP production. Unlike cytosolic ribosomes, mitochondrial ribosomes are specialized for translating mitochondrially encoded mRNAs, which encode 13 subunits of the OXPHOS system. MRPL9. encoded by nuclear DNA, is imported into mitochondria and assembled into the large ribosomal subunit (39S in humans), contributing to ribosome stability and function.
Recombinant MRPL9 protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cell cultures*) by cloning the MRPL9 gene into expression vectors. This allows large-scale production of the protein for functional and structural studies. Recombinant MRPL9 is often tagged (e.g., His-tag, FLAG) to facilitate purification and detection. Its applications include studying mitochondrial ribosome assembly, investigating mutations linked to mitochondrial disorders, and exploring cellular responses to mitochondrial dysfunction.
Dysregulation of MRPL9 is associated with mitochondrial diseases, cancer, and metabolic syndromes. For example, reduced MRPL9 expression impairs mitochondrial translation, leading to OXPHOS deficiencies and cellular energy crises. In cancer, altered MRPL9 levels may influence tumor progression by affecting mitochondrial metabolism. Recombinant MRPL9 also serves as a tool for drug screening, aiming to identify compounds that modulate mitochondrial translation or rescue ribosomal defects.
Overall, MRPL9 recombinant protein is pivotal for advancing research into mitochondrial biology, disease mechanisms, and therapeutic strategies targeting mitochondrial dysfunction.
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