| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | rpmH |
| Uniprot No | P0A7P5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-46aa |
| 氨基酸序列 | MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTVSK |
| 预测分子量 | 32.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于rpmH重组蛋白的3篇模拟参考文献示例(注：内容为虚构，仅用于演示格式)：
1. **文献名称**：Structural characterization of the ribosomal protein L34 (rpmH) from Escherichia coli
**作者**：Kimura T, et al.
**摘要**：本研究解析了大肠杆菌rpmH重组蛋白的晶体结构，揭示了其与23S rRNA结合的特定结构域，并探讨了其在50S核糖体亚基组装中的关键作用。
2. **文献名称**：Functional analysis of rpmH deletion mutants in Bacillus subtilis
**作者**：Zhang L, et al.
**摘要**：通过构建枯草芽孢杆菌rpmH基因敲除株，发现其重组蛋白缺失导致细菌生长迟缓和热应激敏感性增加，表明rpmH在应激响应中具有调控功能。
3. **文献名称**：Heterologous expression and purification of recombinant rpmH for antibiotic target screening
**作者**：Müller F, et al.
**摘要**：开发了一种高效表达纯化rpmH重组蛋白的系统，并基于该蛋白建立高通量筛选模型，用于筛选靶向核糖体组装的小分子抑制剂。
The rpmH gene encodes a ribosomal protein, L34. which is a component of the 50S subunit in prokaryotic ribosomes. As a highly conserved protein across bacterial species, L34 plays a structural and functional role in ribosome assembly and translational fidelity. The rpmH recombinant protein, produced via heterologous expression systems like E. coli, is engineered to study ribosomal biology, antibiotic mechanisms, and bacterial protein synthesis.
Interest in rpmH stems from its potential as a target for antibacterial agents. Ribosomal proteins, including L34. are critical for maintaining ribosome integrity and facilitating interactions between rRNA and translational factors. Recombinant rpmH enables detailed structural analyses (e.g., X-ray crystallography, cryo-EM) to map binding sites for antibiotics or regulatory molecules. Additionally, it serves as a tool to investigate post-translational modifications, protein-rRNA interactions, and ribosome biogenesis in pathogenic bacteria.
The production of rpmH recombinant protein typically involves cloning the gene into expression vectors with affinity tags (e.g., His-tag) for purification. Studies using purified L34 have revealed its role in stabilizing ribosomal architecture and modulating translation under stress conditions. Notably, dysregulation of rpmH expression has been linked to bacterial antibiotic resistance, highlighting its relevance in developing novel therapeutics. Current research also explores its interactions with nascent peptides and chaperones during co-translational folding.
Overall, rpmH recombinant protein provides a molecular platform to dissect fundamental ribosomal processes and identify strategies to disrupt bacterial protein synthesis, offering insights for both basic microbiology and applied drug discovery.
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