| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | rpsK |
| Uniprot No | P0A7R9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-129aa |
| 氨基酸序列 | AKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGWATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNAAGFRITNITDVTPIPHNGCRPPKKRRV |
| 预测分子量 | 17.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于rpsK(核糖体蛋白S11)重组蛋白的模拟参考文献(文献信息为虚构示例，仅供参考格式)：
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1. **文献名称**：*Expression and Purification of Recombinant rpsK in Escherichia coli*
**作者**：Smith A, et al.
**摘要**：报道了通过克隆大肠杆菌rpsK基因至表达载体，优化诱导条件实现高效可溶性表达，并通过亲和层析纯化获得高纯度重组蛋白，用于后续功能研究。
2. **文献名称**：*Functional Analysis of rpsK in Bacterial Stress Response*
**作者**：Chen L, et al.
**摘要**：利用重组rpsK蛋白进行体外结合实验，发现其与特定RNA片段互作，并验证了rpsK在细菌氧化应激中的调控作用，为抗生素靶点研究提供依据。
3. **文献名称**：*Crystallization of Recombinant rpsK from Bacillus subtilis*
**作者**：Wang X, et al.
**摘要**：成功解析枯草芽孢杆菌rpsK重组蛋白的晶体结构(分辨率2.1Å)，揭示其与抗生素结合的关键氨基酸位点，为结构导向药物设计奠定基础。
4. **文献名称**：*rpsK as a Novel Antimicrobial Target: Screening Inhibitors Using Recombinant Protein*
**作者**：Gupta R, et al.
**摘要**：构建重组rpsK的高通量筛选平台，筛选出3种小分子化合物可抑制rpsK的核糖体组装功能，证明其体外抗菌活性。
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**注意**：以上文献为模拟内容，实际研究需通过PubMed、Web of Science或Google Scholar等平台检索关键词(如"rpsK recombinant protein"、"ribosomal protein S11 expression")。真实文献可能涉及原核表达优化、结构功能研究或药物靶点探索等领域。
**Background of RpsK Recombinant Protein**
The RpsK protein, encoded by the *rpsK* gene, is a ribosomal component belonging to the small subunit (30S) of bacterial ribosomes. As part of the S11 ribosomal protein family, RpsK plays a critical role in protein synthesis by stabilizing rRNA structure and facilitating tRNA-mRNA interactions during translation. Its conserved sequence across bacterial species underscores its essential function in cellular viability.
Recombinant RpsK refers to the protein produced via genetic engineering, typically by cloning the *rpsK* gene into expression vectors (e.g., *E. coli*) and purifying the protein using affinity chromatography. This approach allows large-scale production of RpsK for structural and functional studies. Research on recombinant RpsK has been pivotal in understanding ribosome assembly, antibiotic resistance mechanisms, and bacterial pathogenesis. For instance, mutations in *rpsK* have been linked to resistance against aminoglycosides and other antibiotics targeting the 30S subunit.
Additionally, recombinant RpsK serves as a tool for structural biology. Techniques like X-ray crystallography and cryo-EM have utilized the protein to resolve ribosome architecture, revealing interactions between ribosomal proteins, rRNA, and antibiotics. Such insights guide the design of novel antimicrobial agents. Beyond therapeutics, RpsK is explored in synthetic biology for engineering minimal ribosomes and in vaccine development against bacterial pathogens.
Overall, RpsK recombinant protein bridges fundamental microbiology and applied biotechnology, offering avenues to combat antibiotic resistance and develop targeted therapies. Its study remains vital for advancing both basic science and clinical innovation.
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