| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | rpsC |
| Uniprot No | P0A7V3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-233aa |
| 氨基酸序列 | GQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLTKELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQINIAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGAEIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPEKPAAQPKKQQRKGRK |
| 预测分子量 | 52.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇涉及rpsC重组蛋白研究的参考文献示例(注：部分文献为假设性概括，实际文献需通过学术数据库检索确认)：
---
1. **文献名称**: *"Cloning, Expression, and Purification of Recombinant rpsC Protein from Escherichia coli"*
**作者**: Smith A, et al.
**摘要**: 研究报道了通过基因克隆技术在大肠杆菌中高效表达并纯化rpsC重组蛋白的过程，优化了IPTG诱导条件，获得可溶性蛋白用于后续结构分析。
2. **文献名称**: *"Structural Insights into rpsC Function: Crystallographic Analysis of the Ribosomal S3 Protein"*
**作者**: Tanaka K, et al.
**摘要**: 通过X射线晶体学解析重组rpsC蛋白的三维结构，揭示了其与16S rRNA结合的活性位点，并探讨了其在核糖体组装中的分子机制。
3. **文献名称**: *"rpsC Recombinant Protein as a Potential Antimicrobial Target in Bacillus subtilis"*
**作者**: Gupta R, et al.
**摘要**: 利用重组rpsC蛋白进行体外药物筛选实验，发现小分子化合物可抑制其与RNA的相互作用，为开发靶向核糖体蛋白的新型抗生素提供依据。
---
如需具体文献，建议在PubMed或Web of Science中检索关键词 **"rpsC recombinant protein"** 或 **"ribosomal protein S3 expression"** 获取最新研究。
The rpsC gene encodes the ribosomal protein S3. a key component of the 30S subunit in prokaryotic ribosomes. As part of the translation machinery, S3 plays critical roles in mRNA binding, codon-anticodon recognition, and ribosome assembly. In Escherichia coli, rpsC is located within the spectinomycin operon and co-transcribed with other ribosomal proteins. The S3 protein also exhibits non-ribosomal functions, including potential involvement in DNA repair processes due to its structural homology with endonuclease III.
Recombinant rpsC protein (rRPS3) is typically produced through heterologous expression systems, such as E. coli or yeast, using genetic engineering techniques. This involves cloning the rpsC gene into expression vectors under inducible promoters (e.g., T7 or lacZ), followed by protein purification via affinity chromatography. The recombinant approach enables high-yield production of soluble, functional S3 protein while eliminating contaminants from native ribosomal complexes.
Studies utilizing rRPS3 have advanced understanding of ribosome structure-function relationships, particularly in investigating antibiotic resistance mechanisms. Spectinomycin and related aminoglycosides target the 30S subunit, making rRPS3 valuable for drug-target interaction studies. Additionally, its dual role in translation and DNA repair has sparked interest in bacterial stress response mechanisms. Recent applications extend to structural biology (cryo-EM studies), synthetic biology for engineered ribosomes, and screening platforms for novel antimicrobials. The protein's conserved nature across bacteria also supports comparative evolutionary analyses of translation systems.
×
