| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | rpsD |
| Uniprot No | P0A7V8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-206aa |
| 氨基酸序列 | ARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGARKPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVVYRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALELAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK |
| 预测分子量 | 27.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于rpsD重组蛋白的3篇虚构参考文献及其摘要概括，供参考：
1. **文献名称**：Cloning and Expression of Recombinant rpsD in Escherichia coli
**作者**：M. Thompson et al.
**摘要**：本研究成功克隆并表达了rpsD基因编码的核糖体蛋白S4.利用大肠杆菌表达系统获得高纯度重组蛋白。通过SDS-PAGE和质谱验证蛋白分子量，并证实其与16S rRNA的结合活性，为后续功能研究奠定基础。
2. **文献名称**：Structural Analysis of rpsD Mutants in Ribosome Assembly
**作者**：K. Suzuki & T. Yamamoto
**摘要**：通过X射线晶体学解析rpsD突变体重组蛋白的结构，发现其C端结构域对30S亚基组装至关重要。突变导致rRNA结合能力下降，揭示了rpsD在核糖体正确折叠中的关键作用。
3. **文献名称**：Functional Characterization of Recombinant rpsD in Antibiotic Sensitivity
**作者**：L. Chen et al.
**摘要**：研究发现过表达重组rpsD蛋白可增强大肠杆菌对链霉素的耐受性。通过荧光偏振实验证实rpsD与抗生素竞争性结合核糖体，提出了其在耐药性中的潜在调控机制。
注：以上文献为示例性质，实际研究中建议通过PubMed或Web of Science等平台检索真实发表的论文。
The rpsD gene encodes the ribosomal protein S4. a critical component of the 30S ribosomal subunit in bacteria, notably Escherichia coli. As part of the ribosome, S4 plays a pivotal role in translation initiation and accuracy by binding to 16S rRNA, stabilizing its structure, and ensuring proper assembly of the ribosomal subunit. Beyond its structural role, S4 also functions as a translational repressor, regulating the expression of the α-operon (S10 ribosomal protein cluster) through feedback mechanisms. This dual functionality underscores its importance in maintaining cellular protein synthesis homeostasis.
Recombinant rpsD protein is typically produced via heterologous expression systems, such as E. coli, using plasmid vectors under inducible promoters (e.g., T7 or lac). The recombinant protein often includes affinity tags (e.g., His-tag) for simplified purification. Its production enables detailed biochemical and structural studies, including X-ray crystallography and cryo-EM, to elucidate ribosome assembly mechanisms and S4's interaction with rRNA or antibiotics.
Research on recombinant S4 has implications for understanding antibiotic resistance, as mutations in rpsD can confer resistance to streptomycin by altering ribosome-drug interactions. Additionally, it serves as a tool for studying ribosomal diseases and developing novel antimicrobials targeting ribosome biogenesis. In synthetic biology, engineered S4 variants help probe ribosome adaptability and engineer specialized translation systems. Overall, rpsD recombinant protein bridges fundamental ribosome biology with practical applications in drug discovery and genetic engineering.
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