| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | rpsR |
| Uniprot No | P0A7T7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-75aa |
| 氨基酸序列 | ARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRITGTRAKYQRQLARAIKRARYLSLLPYTDRHQ |
| 预测分子量 | 35.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于rpsR重组蛋白的3篇参考文献示例(内容基于假设性研究，实际文献需自行验证)：
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1. **文献名称**: *Expression and Purification of Recombinant rpsR Protein in Escherichia coli*
**作者**: Smith A, et al.
**摘要**: 本研究报道了通过克隆结核分枝杆菌rpsR基因至大肠杆菌表达系统，优化诱导条件并利用亲和层析技术纯化重组rpsR蛋白。结果表明，重组蛋白可溶性表达且纯度达95%，为后续功能研究奠定基础。
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2. **文献名称**: *Structural Characterization of rpsR in Ribosome Assembly*
**作者**: Zhang Y, et al.
**摘要**: 通过X射线晶体学解析了重组rpsR蛋白的三维结构，揭示其与核糖体16S rRNA的结合位点，阐明rpsR在细菌核糖体小亚基组装中的关键作用。
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3. **文献名称**: *Functional Analysis of rpsR in Bacterial Stress Response*
**作者**: Lee H, et al.
**摘要**: 研究发现重组rpsR蛋白与细菌氧化应激反应相关蛋白(如SoxS)存在相互作用，敲除rpsR后细菌对氧化压力耐受性下降，提示其潜在调控应激通路的功能。
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注：以上文献为示例，实际引用时请以真实发表的论文为准，并核实作者、标题及摘要内容。
The rpsR gene encodes the ribosomal protein S18. a conserved component of the small (30S) subunit in bacterial ribosomes. As part of the translation machinery, S18 plays a critical role in protein synthesis by stabilizing rRNA structure and facilitating ribosome assembly. In recombinant protein production, rpsR-derived S18 is often expressed in heterologous systems like *E. coli* for structural and functional studies. Its compact, RNA-binding domain-rich structure makes it a model for investigating ribosome biogenesis and antibiotic interactions.
Interest in recombinant S18 stems from its implications in bacterial pathogenesis and antimicrobial resistance. For example, in *Mycobacterium tuberculosis*, RpsR interacts with host immune components, suggesting potential as a diagnostic or therapeutic target. Structural analyses (e.g., X-ray crystallography) of recombinant S18 have revealed binding sites for antibiotics like spectinomycin, informing drug design. Additionally, engineered S18 variants help dissect ribosome function in genetic code expansion research.
Beyond basic science, recombinant RpsR serves as an antigen in serodiagnostics for bacterial infections and a tool for studying stress responses linked to ribosomal hibernation. Its conservation across species also aids evolutionary studies of ribosomal adaptation. Despite its small size (~9 kDa), S18's multifaceted roles underscore its importance in both fundamental microbiology and applied biotechnology.
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