| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | rpsU |
| Uniprot No | P68679 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-71aa |
| 氨基酸序列 | PVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTERKRAKASAVKRHAKKLARENARRTRLY |
| 预测分子量 | 35.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与rpsU重组蛋白相关的代表性文献(内容基于真实研究方向概括，部分信息为模拟整合)：
1. **"Cloning and expression of the rpsU gene in Escherichia coli: Role of ribosomal protein S21 in stress adaptation"**
- **作者**: Smith, J.R., et al.
- **摘要**: 研究通过克隆大肠杆菌rpsU基因并表达重组S21蛋白，发现该蛋白在氧化应激和热休克反应中调控核糖体稳定性，缺失突变体表现出生长迟缓和应激敏感性增加。
2. **"Structural characterization of recombinant RpsU from Bacillus subtilis and its interaction with RNA"**
- **作者**: Lee, H., & Kim, M.
- **摘要**: 解析枯草芽孢杆菌rpsU重组蛋白的晶体结构，揭示其与16S rRNA的特定区域结合，为阐明S21蛋白在翻译起始中的分子机制提供结构基础。
3. **"Optimization of recombinant RpsU production in Pseudomonas aeruginosa for antibiotic susceptibility studies"**
- **作者**: Gonzalez, A., et al.
- **摘要**: 通过优化表达条件(如诱导温度、IPTG浓度)提高铜绿假单胞菌RpsU蛋白的溶解度，并证明其过表达可增强细菌对氨基糖苷类抗生素的敏感性。
4. **"Functional analysis of Mycobacterium tuberculosis RpsU in host-pathogen interactions"**
- **作者**: Patel, S., & Dasgupta, S.
- **摘要**: 利用重组结核分枝杆菌RpsU蛋白进行宿主巨噬细胞感染实验，发现其通过调控细菌胞内存活相关通路，可能作为潜在抗结核药物靶点。
注：以上文献标题和内容为领域典型研究方向模拟，实际文献需通过PubMed/Google Scholar以关键词“rpsU recombinant protein”或“ribosomal protein S21”检索确认。
The rpsU gene encodes the 30S ribosomal subunit protein S21. a small but essential component of bacterial ribosomes. In *Escherichia coli* and other bacteria, rpsU is part of a conserved genomic region often linked to stress-response pathways. It is co-transcribed with genes like uvrB (involved in DNA repair) and ssb (single-stranded DNA-binding protein) in some species, forming an operon that integrates translational machinery with DNA damage and oxidative stress responses. This arrangement suggests a regulatory connection between ribosome biogenesis and cellular stress adaptation.
The S21 protein plays a critical role in ribosome assembly and translation initiation, particularly in recognizing mRNA start codons. Studies show that rpsU expression is tightly regulated under varying conditions, including nutrient deprivation, heat shock, and antibiotic exposure. Its promoter region contains binding sites for global regulators like LexA (linked to the SOS response) and stringent response mediators, highlighting its role in stress adaptation.
Recombinant rpsU protein (rRpsU) is typically produced via heterologous expression in *E. coli* systems for structural and functional studies. Purified rRpsU has been used to investigate ribosome assembly mechanisms, protein-RNA interactions, and its auxiliary roles in DNA repair processes. Notably, rpsU deletion or mutations impair bacterial growth and stress tolerance, making it a potential target for antimicrobial strategies. Research on rRpsU also contributes to understanding conserved ribosome biogenesis pathways across prokaryotes and eukaryotes, offering insights into evolutionary parallels in translation regulation.
Overall, rpsU serves as a model for studying interconnected translational and stress-response networks, with recombinant protein tools enabling detailed mechanistic explorations.
×
