| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | G3BP2 |
| Uniprot No | Q9UN86 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-449aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSHMVMEKP SPLLVGREFV RQYYTLLNKA PEYLHRFYGR NSSYVHGGVD ASGKPQEAVY GQNDIHHKVL SLNFSECHTK IRHVDAHATL SDGVVVQVMG LLSNSGQPER KFMQTFVLAP EGSVPNKFYV HNDMFRYEDE VFGDSEPELD EESEDEVEEE QEERQPSPEP VQENANSGYY EAHPVTNGIE EPLEESSHEP EPEPESETKT EELKPQVEEK NLEELEEKST TPPPAEPVSL PQEPPKPRVE AKPEVQSQPP RVREQRPRER PGFPPRGPRP GRGDMEQNDS DNRRIIRYPD SHQLFVGNLP HDIDENELKE FFMSFGNVVE LRINTKGVGG KLPNFGFVVF DDSEPVQRIL IAKPIMFRGE VRLNVEEKKT RAARERETRG GGDDRRDIRR NDRGPGGPRG IVGGGMMRDR DGRGPPPRGG MAQKLGSGRG TGQMEGRFTG QRR |
| 预测分子量 | 53 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于G3BP2重组蛋白的相关文献概览(基于近年研究主题)：
1. **文献名称**：*"G3BP2 is required for the proliferation and survival of breast cancer cells"*
**作者**：Li P. et al.
**摘要**：该研究利用重组人G3BP2蛋白进行体外细胞实验，发现G3BP2通过激活mTORC1信号通路促进乳腺癌细胞增殖，并证明其表达水平与患者预后不良相关。
2. **文献名称**：*"Structural insights into G3BP2-mediated stress granule assembly"*
**作者**：Yang W. et al.
**摘要**：通过重组表达纯化G3BP2蛋白，结合冷冻电镜技术解析了其与RNA及互作蛋白的复合物结构，揭示了G3BP2在应激颗粒形成中的构象变化和功能机制。
3. **文献名称**：*"G3BP2 interacts with RAS signaling components to regulate colorectal cancer metastasis"*
**作者**：Zhang Y. et al.
**摘要**：利用重组G3BP2蛋白进行体外结合实验，发现其与KRAS蛋白直接互作，并通过调控MAPK信号通路促进结直肠癌转移，为靶向治疗提供新靶点。
注：以上文献为示例性内容，实际文献需通过PubMed或Web of Science等平台检索确认(关键词：G3BP2. recombinant protein, stress granule, cancer signaling)。近期研究多聚焦于G3BP2在肿瘤、神经退行性疾病中的作用及其相分离机制。
G3BP2 (G3BP stress granule assembly factor 2) is a multifunctional RNA-binding protein belonging to the G3BP family, which also includes its closely related paralog G3BP1. Structurally, G3BP2 contains an N-terminal nuclear transport factor 2 (NTF2)-like domain responsible for dimerization and interaction with ubiquitin-specific proteases, followed by RNA recognition motifs (RRMs) and arginine-glycine-rich (RGG) regions that mediate RNA binding. It plays a pivotal role in cellular stress responses by nucleating stress granules (SGs), dynamic cytoplasmic condensates formed during environmental stresses (e.g., heat shock, oxidative stress, viral infection) to transiently sequester untranslated mRNAs and signaling molecules.
Beyond stress granule dynamics, G3BP2 participates in regulating mRNA stability, translation, and decay. It interacts with components of the Ras-GAP SH3 domain-binding network and modulates multiple signaling pathways, including NF-κB and MAPK cascades. Dysregulation of G3BP2 has been implicated in cancer progression (e.g., promoting metastasis in breast and liver cancers), neurodegenerative diseases (e.g., by influencing TDP-43 or FUS aggregation), and viral pathogenesis (e.g., facilitating viral replication by SG disruption).
Recombinant G3BP2 proteins, typically produced in *E. coli* or mammalian expression systems with affinity tags (e.g., His, GST), enable *in vitro* studies of its RNA-binding properties, protein interactions, and SG assembly mechanisms. These tools are critical for screening therapeutic compounds targeting G3BP2-mediated pathways or stress granule-associated pathologies. Recent studies also explore engineered G3BP2 variants to dissect domain-specific functions or develop synthetic SG-targeting systems for biotechnology applications. Its dual role in cell survival and death pathways continues to make G3BP2 a compelling subject for both basic research and drug discovery.
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