| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GCAT |
| Uniprot No | O75600 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22-419aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMSALAQLRGILEGELEGIRGAGTWKSERVI TSRQGPHIRVDGVSGGILNFCANNYLGLSSHPEVIQAGLQALEEFGAGLS SVRFICGTQSIHKNLEAKIARFHQREDAILYPSCYDANAGLFEALLTPED AVLSDELNHASIIDGIRLCKAHKYRYRHLDMADLEAKLQEAQKHRLRLVA TDGAFSMDGDIAPLQEICCLASRYGALVFMDECHATGFLGPTGRGTDELL GVMDQVTIINSTLGKALGGASGGYTTGPGPLVSLLRQRARPYLFSNSLPP AVVGCASKALDLLMGSNTIVQSMAAKTQRFRSKMEAAGFTISGASHPICP VMLGDARLASRMADDMLKRGIFVIGFSYPVVPKGKARIRVQISAVHSEED IDRCVEAFVEVGRLHGALP |
| 预测分子量 | 45 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GCAT重组蛋白的假设性参考文献示例(仅供参考，非真实文献)：
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1. **文献名称**：*Heterologous Expression and Characterization of GCAT from Neisseria meningitidis*
**作者**：Smith A, et al.
**摘要**：研究在大肠杆菌中重组表达脑膜炎奈瑟菌GCAT(甘油磷酸胆碱酰基转移酶)，通过亲和层析纯化蛋白，并验证其催化磷脂合成的酶活性，为疫苗开发提供候选抗原。
2. **文献名称**：*Structural Insights into GCAT Enzyme via Recombinant Protein Crystallography*
**作者**：Johnson B, et al.
**摘要**：利用昆虫细胞系统表达重组GCAT蛋白，解析其晶体结构，揭示底物结合位点及催化机制，为针对细菌感染的抑制剂设计提供结构基础。
3. **文献名称**：*GCAT as a Virulence Factor: Functional Analysis Using Recombinant Mutants*
**作者**：Lee C, et al.
**摘要**：通过构建GCAT重组突变体，证明其参与病原菌毒力，小鼠模型显示敲除GCAT显著降低细菌存活率，提示其作为治疗靶点的潜力。
4. **文献名称**：*Optimization of GCAT Production in Pichia pastoris for Industrial Applications*
**作者**：Wang D, et al.
**摘要**：在毕赤酵母中优化GCAT重组表达条件，实现高效分泌表达，产物在生物催化合成中展现高稳定性，适用于规模化工业生产。
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注：以上为模拟文献，实际引用需查询PubMed、Google Scholar等平台，关键词“GCAT recombinant protein”或结合具体物种/功能(如“Neisseria GCAT”)检索。
GCAT recombinant protein is a genetically engineered biomolecule derived from the GCAT (Glycerophospholipid:Cholesterol Acyltransferase) enzyme, which plays a critical role in lipid metabolism and bacterial pathogenesis. Originally identified in pathogenic bacteria such as *Streptococcus pneumoniae* and *Mycoplasma species*, GCAT catalyzes the transfer of fatty acid chains from phospholipids to cholesterol, generating cholesteryl esters and lysophospholipids. This enzymatic activity contributes to bacterial virulence by disrupting host cell membranes, evading immune responses, and facilitating colonization.
Recombinant GCAT is produced via heterologous expression systems, typically using *E. coli* or yeast as host organisms. The gene encoding GCAT is cloned into expression vectors, allowing large-scale production and purification. This engineered protein retains the functional domains of native GCAT but is often modified with affinity tags (e.g., His-tag) to streamline purification processes. Structural studies of recombinant GCAT have revealed its conserved α/β-hydrolase fold and catalytic triad, which are essential for enzymatic activity.
Research on GCAT recombinant protein focuses on its dual role as both a virulence factor and a potential therapeutic target. In vaccine development, it serves as an antigen to induce immune responses against bacterial infections. Additionally, its membrane-remodeling properties are explored for industrial applications, including lipid modification and biosurfactant production. Recent studies also investigate its interaction with host cells to uncover mechanisms of bacterial persistence and antibiotic resistance. Overall, GCAT recombinant protein is a versatile tool for advancing microbiology, immunology, and biotechnology research.
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