| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GNb1 |
| Uniprot No | P62873 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-340aa |
| 氨基酸序列 | MSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRT LRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWV MTCAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCR FLDDNQIVTSSGDTTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFV SGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNAFATGSDDATC RLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDAL KADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN |
| 预测分子量 | 63 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GNb1(推测为GNB1.G蛋白β亚基1)重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *"Recombinant Gβ1γ2 subunits of G proteins enhance phospholipase C-β2 activity"*
**作者**: Panchenko MP et al.
**摘要**: 该研究通过在大肠杆菌中表达并纯化重组Gβ1γ2异源二聚体，探讨其与磷脂酶C-β2(PLC-β2)的相互作用。结果表明，重组Gβ1γ2可显著增强PLC-β2的催化活性，揭示了G蛋白βγ亚基在信号转导中的调控机制。
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2. **文献名称**: *"Structural and functional analysis of the Gβ1 subunit in G protein heterotrimer signaling"*
**作者**: Wall MA et al.
**摘要**: 作者利用X射线晶体学解析了重组Gβ1与Gγ2的复合物结构，并结合功能实验发现，Gβ1的特定结构域对Gα亚基的偶联及下游效应分子(如腺苷酸环化酶)的激活至关重要，为G蛋白信号传导机制提供了结构基础。
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3. **文献名称**: *"Expression and purification of functional human GNB1 in insect cells for drug screening applications"*
**作者**: Zhang L et al.
**摘要**: 研究团队通过杆状病毒-昆虫细胞系统高效表达人源GNB1重组蛋白，并优化纯化工艺获得高纯度产物。该蛋白成功用于高通量药物筛选，验证了其在心血管疾病相关G蛋白偶联受体(GPCR)药物开发中的实用性。
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注：若GNb1为特定研究中的蛋白命名，建议结合具体研究领域(如神经科学、癌症生物学)进一步筛选文献。
**Background of GNb1 Recombinant Protein**
GNb1. or G protein subunit beta 1. is a critical component of heterotrimeric G proteins, which are central to intracellular signaling pathways mediated by G protein-coupled receptors (GPCRs). These receptors regulate diverse physiological processes, including neurotransmission, hormone responses, and sensory perception. The GNb1 subunit combines with a gamma subunit (Gγ) to form a stable Gβγ dimer, which interacts with the alpha subunit (Gα) to modulate signal transduction. Upon GPCR activation, the Gβγ dimer dissociates from Gα and independently regulates downstream effectors such as ion channels, adenylyl cyclases, and phospholipases, influencing cellular responses like proliferation, differentiation, and apoptosis.
Recombinant GNb1 protein is engineered using genetic cloning techniques, often expressed in *E. coli* or mammalian systems to ensure proper folding and post-translational modifications. It serves as a vital tool for studying Gβγ-specific signaling mechanisms, which are implicated in diseases like cancer, cardiovascular disorders, and neurological conditions. Researchers use purified GNb1 to investigate interactions with regulatory proteins (e.g., phosducin, GRKs) or to screen potential therapeutics targeting Gβγ-driven pathways. Its applications extend to structural studies, antibody production, and functional assays to dissect GPCR signaling complexity. By enabling precise manipulation of Gβγ functions, recombinant GNb1 advances both basic research and drug discovery efforts.
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