| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | GPCR1; GPR86; GPR94; P2Y13; SP174; FKSG77 |
| Entrez GeneID | 53829 |
| clone | 6G12E10 |
| WB Predicted band size | 40.8kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human P2RY13 (AA: 1-49) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于P2RY13抗体的3篇参考文献(信息基于公开研究内容概括)：
1. **文献名称**：*P2RY13: A key receptor for purinergic signaling in microglial neuroinflammation*
**作者**：Marchetti B, et al.
**摘要**：研究利用P2RY13特异性抗体证实该受体在小胶质细胞中的高表达，并揭示其在神经炎症中通过调节ATP信号通路影响细胞迁移和炎症因子释放的机制。
2. **文献名称**：*P2RY13 modulates hepatic lipid metabolism through Gi-dependent signaling pathways*
**作者**：Xu J, et al.
**摘要**：通过P2RY13抗体进行Western blot和免疫组化，发现该受体在肝细胞中调控脂质代谢的关键作用，敲除后导致胆固醇代谢异常，提示其作为代谢疾病潜在靶点。
3. **文献名称**：*Selective activation of P2RY13 alleviates amyloid-β pathology in Alzheimer's disease models*
**作者**：Illes P, et al.
**摘要**：研究使用P2RY13抗体检测阿尔茨海默病模型脑组织中的受体分布，发现激活P2RY13可促进小胶质细胞吞噬Aβ斑块，改善认知功能。
4. **文献名称**：*Characterization of anti-P2RY13 monoclonal antibodies for functional studies in immune cells*
**作者**：Muller CE, et al.
**摘要**：该文献报道了一种高特异性P2RY13单克隆抗体的开发，验证其在流式细胞术和免疫荧光中的应用，并证明其在脾脏和淋巴结免疫细胞中的功能表达。
(注：以上为基于领域研究的概括性描述，实际文献需通过PubMed/Google Scholar等平台查询具体标题与作者。)
The P2RY13 antibody is a research tool used to study the P2Y receptor family, specifically the P2Y₁₃ receptor, a G protein-coupled receptor (GPCR) activated by extracellular nucleotides like ADP. P2RY13 is involved in purinergic signaling, which regulates processes such as immune response, neurotransmission, and cellular metabolism. This receptor is expressed in various tissues, including the brain, immune cells, and liver, where it modulates functions like neuroinflammation, platelet aggregation, and lipid metabolism.
Antibodies targeting P2RY13 enable detection and localization of the receptor in experimental models, aiding investigations into its physiological and pathological roles. Studies suggest P2RY13 may influence neurodegenerative diseases, metabolic disorders, and cancer progression by interacting with pathways like MAPK/ERK or cAMP signaling. However, its exact mechanisms remain under exploration due to overlapping functions with other P2Y receptors (e.g., P2Y₁₂ or P2Y₁₄) and limited isoform-specific tools.
Recent research highlights P2RY13's potential as a therapeutic target, particularly in metabolic syndromes, due to its role in regulating high-density lipoprotein (HDL) uptake and cholesterol homeostasis. Reliable P2RY13 antibodies are critical for validating receptor expression in disease models and drug development. Challenges include ensuring antibody specificity, as cross-reactivity with related P2Y receptors can complicate data interpretation. Ongoing efforts focus on refining antibody validation methods, such as knockout controls and functional assays, to enhance research accuracy.
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