| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | PP4; ANX5; ENX2; RPRGL3; HEL-S-7 |
| Entrez GeneID | 308 |
| clone | 3B9B7 |
| WB Predicted band size | 36kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Purified recombinant fragment of human ANXA5 (AA: 160-320) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于ANXA5抗体的3篇代表性文献，涵盖不同研究领域：
1. **文献名称**：*"Anti-annexin A5 antibodies in women with recurrent pregnancy loss"*
**作者**：Bardin N. et al.
**摘要**：研究发现复发性流产(RPL)患者血清中抗ANXA5抗体水平显著升高，可能通过干扰ANXA5的抗凝血功能，导致胎盘血栓形成或滋养层功能异常。
2. **文献名称**：*"Annexin A5 autoantibodies in antiphospholipid syndrome"*
**作者**：Rand J.H. et al.
**摘要**：探讨抗磷脂综合征(APS)患者中ANXA5抗体的存在与病理机制的关系，提出抗体可能破坏ANXA5在细胞表面的抗凝血屏障作用，加剧血栓形成风险。
3. **文献名称**：*"The role of anti-annexin A5 antibodies in systemic lupus erythematosus"*
**作者**：Satoh A. et al.
**摘要**：系统性红斑狼疮(SLE)患者中ANXA5抗体与疾病活动度相关，可能通过干扰细胞凋亡清除过程，促进自身抗原释放和炎症反应。
4. **文献名称**：*"Annexin A5 as a novel target in cancer immunotherapy"*
**作者**：Gong Y. et al.
**摘要**：研究ANXA5抗体在肿瘤微环境中的作用，发现其可能通过阻断ANXA5介导的免疫抑制信号，增强T细胞抗肿瘤活性，为癌症治疗提供新思路。
以上文献覆盖了ANXA5抗体在生殖健康、自身免疫病及肿瘤领域的病理机制和潜在应用。
ANXA5 (Annexin A5) is a calcium-dependent phospholipid-binding protein involved in cellular processes like apoptosis, membrane repair, and anticoagulation. It binds to phosphatidylserine exposed on apoptotic cell surfaces, preventing pro-inflammatory responses and thrombotic reactions. ANXA5 antibodies are tools used in research and diagnostics to detect the presence, localization, or functional alterations of ANXA5 in biological samples. These antibodies are generated in animal models (e.g., mice, rabbits) using purified recombinant ANXA5 or peptide fragments as immunogens.
In clinical contexts, ANXA5 autoantibodies have been linked to conditions such as recurrent pregnancy loss, antiphospholipid syndrome (APS), and thrombosis, as they may disrupt ANXA5’s anticoagulant shield on placental or vascular surfaces. Research applications include studying apoptosis in cancer, cardiovascular diseases, and autoimmune disorders. Monoclonal ANXA5 antibodies are valued for specificity, while polyclonal versions offer broader epitope recognition. Detection methods like ELISA, Western blot, and immunohistochemistry rely on these antibodies to quantify ANXA5 expression or assess its role in disease models. However, variability in antibody specificity and cross-reactivity with other annexins requires careful validation. Current studies explore ANXA5 antibodies as potential therapeutic agents or biomarkers for disease progression.
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