| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | CXXC2; Fbl10; PCCX2; FBXL10; JHDM1B |
| Entrez GeneID | 84678 |
| clone | 3B7D11 |
| WB Predicted band size | 152.6kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human FBXL10 (AA: 457-555) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于FBXL10抗体的3篇参考文献示例(文献信息为虚拟概括，仅作参考)：
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1. **文献名称**：*FBXL10 regulates polycomb-mediated chromatin modifications through ubiquitination in embryonic stem cells*
**作者**：He J, et al.
**摘要**：本研究利用FBXL10特异性抗体进行染色质免疫沉淀(ChIP)，揭示了FBXL10通过招募Cullin-RING泛素连接酶复合物，调控多梳蛋白(Polycomb)介导的组蛋白H2A泛素化，从而维持胚胎干细胞的自我更新能力。
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2. **文献名称**：*KDM2B/FBXL10 demethylates H3K36me3 to facilitate cellular senescence*
**作者**：Frescas D, et al.
**摘要**：通过FBXL10抗体的Western blot和免疫荧光实验，研究者发现FBXL10通过去甲基化H3K36me3修饰，激活衰老相关基因表达，并证实其在细胞衰老过程中的表观遗传调控作用。
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3. **文献名称**：*FBXL10 interacts with p53 to suppress tumorigenesis via modulating ribosomal RNA transcription*
**作者**：Blackledge NP, et al.
**摘要**：使用FBXL10抗体进行免疫共沉淀(Co-IP)和蛋白质互作分析，证明FBXL10与肿瘤抑制蛋白p53直接结合，共同抑制核糖体RNA的转录，从而抑制肿瘤发生。
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4. **文献名称**：*Structural and functional analysis of the FBXL10-SKP1 complex in chromatin remodeling*
**作者**：Tsukada Y, et al.
**摘要**：通过FBXL10抗体的体外Pull-down实验，解析了FBXL10与SKP1蛋白的相互作用结构域，并阐明其通过SCF泛素化复合物调控染色质重塑的分子机制。
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(注：上述文献为示例性概括，实际引用时需核实真实文献信息。)
FBXL10 (F-box and leucine-rich repeat protein 10), also known as JHDM1B or KDM2B, is a member of the F-box protein family that plays a role in ubiquitin-mediated proteolysis by forming SCF (Skp1-Cul1-F-box) E3 ubiquitin ligase complexes. It contains an F-box domain for Skp1 binding and leucine-rich repeats (LRRs) for substrate interaction, enabling substrate recognition and degradation. Additionally, FBXL10 functions as a histone demethylase, targeting H3K36me2/me1 through its JmjC domain, thereby regulating gene expression and epigenetic silencing.
FBXL10 antibodies are essential tools for studying its dual role in protein ubiquitination and chromatin remodeling. These antibodies are used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to detect FBXL10 expression, localization, and interactions. Researchers employ them to explore FBXL10's involvement in cellular processes such as cell cycle regulation, differentiation, and tumorigenesis. Studies have linked FBXL10 to both oncogenic and tumor-suppressive activities, depending on cellular context, making it a focus in cancer research.
Antibody specificity is critical due to FBXL10’s splice variants (e.g., FBXL10-1 and FBXL10-2) and post-translational modifications. Validated antibodies help distinguish its roles in recruiting Polycomb repressive complexes or degrading substrates like p53. FBXL10 antibodies also aid in dissecting its tissue-specific functions, particularly in stem cells and developmental models. Overall, these antibodies are vital for unraveling FBXL10's complex regulatory networks in health and disease.
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