| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | cry2Aa |
| Uniprot No | P0A377 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-633aa |
| 氨基酸序列 | MNNVLNSGRTTICDAYNVVAHDPFSFEHKSLDTIQKEWMEWKRTDHSLYVAPVVGTVSSFLLKKVGSLIGKRILSELWGIIFPSGSTNLMQDILRETEQFLNQRLNTDTLARVNAELIGLQANIREFNQQVDNFLNPTQNPVPLSITSSVNTMQQLFLNRLPQFQIQGYQLLLLPLFAQAANMHLSFIRDVILNADEWGISAATLRTYRDYLRNYTRDYSNYCINTYQTAFRGLNTRLHDMLEFRTYMFLNVFEYVSIWSLFKYQSLMVSSGANLYASGSGPQQTQSFTAQNWPFLYSLFQVNSNYILSGISGTRLSITFPNIGGLPGSTTTHSLNSARVNYSGGVSSGLIGATNLNHNFNCSTVLPPLSTPFVRSWLDSGTDREGVATSTNWQTESFQTTLSLRCGAFSARGNSNYFPDYFIRNISGVPLVIRNEDLTRPLHYNQIRNIESPSGTPGGARAYLVSVHNRKNNIYAANENGTMIHLAPEDYTGFTISPIHATQVNNQTRTFISEKFGNQGDSLRFEQSNTTARYTLRGNGNSYNLYLRVSSIGNSTIRVTINGRVYTVSNVNTTTNNDGVNDNGARFSDINIGNIVASDNTNVTLDINVTLNSGTPFDLMNIMFVPTNLPPLY |
| 预测分子量 | 70,8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Cry2Aa重组蛋白的3篇参考文献示例(虚构内容，仅供格式参考)：
1. **文献名称**: *Expression and purification of recombinant Cry2Aa toxin in Escherichia coli*
**作者**: Zhang, L., Wang, Y., & Li, J.
**摘要**: 研究报道了通过大肠杆菌表达系统高效表达Cry2Aa蛋白的优化方法，采用亲和层析纯化获得高纯度蛋白，并验证其对棉铃虫幼虫的杀虫活性。
2. **文献名称**: *Structural and functional analysis of Cry2Aa toxin domain interactions*
**作者**: Kumar, S., et al.
**摘要**: 通过X射线晶体学解析Cry2Aa蛋白的三维结构，揭示其结构域结合机制，并证明特定氨基酸位点对昆虫中肠受体结合的关键作用。
3. **文献名称**: *Synergistic insecticidal effects of Cry2Aa with other Bt toxins*
**作者**: Fernández-Luna, M.T., et al.
**摘要**: 评估Cry2Aa与Cry1Ac蛋白的协同作用，发现联合使用可显著增强对抗性小菜蛾的杀虫效果，为延缓害虫抗性发展提供策略。
(注：以上为模拟内容，实际文献需通过学术数据库检索。)
Cry2Aa is a recombinant protein derived from the Bacillus thuringiensis (Bt) bacterium, a well-known source of insecticidal toxins widely used in agricultural biotechnology. As a member of the Cry toxin family, Cry2Aa exhibits specific toxicity against lepidopteran pests, such as cotton bollworms and armyworms, which cause significant damage to crops. Unlike other Cry proteins (e.g., Cry1Ab), Cry2Aa binds to distinct receptors on insect midgut epithelial cells, forming pores that disrupt membrane integrity, leading to cell lysis and eventual insect death. This unique mode of action makes it valuable for integrated pest management, particularly in delaying insect resistance when combined with other Cry toxins.
Recombinant Cry2Aa is produced through genetic engineering, where the cry2Aa gene is cloned and expressed in heterologous systems like Escherichia coli or yeast. This allows large-scale production of the protein for use in biopesticides or transgenic crops. Its stability under field conditions and specificity to target pests enhance its appeal as an environmentally friendly alternative to chemical insecticides, minimizing harm to non-target organisms and reducing ecological disruption.
Research on Cry2Aa also focuses on optimizing its efficacy and safety. Studies explore structural modifications to improve binding affinity or expand its target range while maintaining low mammalian toxicity. Additionally, Cry2Aa has been incorporated into stacked Bt crops (e.g., cotton and maize) alongside other Cry or Vip toxins, providing multi-mechanistic pest control. This strategy not only boosts crop yields but also aligns with sustainable agriculture practices by reducing pesticide overuse. Despite its advantages, challenges such as potential resistance evolution in pests and regulatory hurdles for transgenic applications remain areas of active investigation. Overall, Cry2Aa exemplifies the intersection of microbial biology and biotechnology in addressing global food security challenges.
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