Mouse Monoclonal CBX5 Antibody

The CBX5 antibody is a widely used tool in epigenetics research, targeting the Chromobox Homolog 5 (CBX5) protein, also known as Heterochromatin Protein 1α (HP1α). CBX5 is a member of the Polycomb group (PcG) protein family and plays a critical role in heterochromatin formation, gene silencing, and chromatin organization. It contains a conserved chromodomain that binds to trimethylated lysine 9 on histone H3 (H3K9me3), a hallmark of transcriptionally repressive heterochromatin. Researchers utilize CBX5 antibodies in techniques like chromatin immunoprecipitation (ChIP), immunofluorescence, and Western blotting to study its localization, interactions, and expression across cell types. These studies have linked CBX5 to diverse biological processes, including DNA repair, cell cycle regulation, and X-chromosome inactivation. Dysregulation of CBX5 has been implicated in cancer progression, aging-related diseases, and developmental disorders, making it a focus of therapeutic exploration. The antibody's specificity is crucial for distinguishing CBX5 from other HP1 isoforms (HP1β/CBX1 and HP1γ/CBX3), which share structural similarities but exhibit functional divergence. Recent applications extend to single-cell genomics and CRISPR-based screens, underscoring its relevance in advancing chromatin biology and epigenetic drug discovery.