| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | PFM1 |
| Entrez GeneID | 11108 |
| clone | 4B6G1 |
| WB Predicted band size | 88kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human PRDM4 (AA: 476-575) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是假设性的参考文献示例，供参考(建议通过PubMed或Google Scholar获取最新真实文献)：
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1. **文献名称**：*PRDM4 regulates epigenetic silencing of tumor suppressor genes in neuroblastoma*
**作者**：Smith J, et al.
**摘要**：研究利用PRDM4抗体通过ChIP-seq和Western blot技术，揭示PRDM4通过招募组蛋白去乙酰化酶(HDACs)沉默肿瘤抑制基因，促进神经母细胞瘤进展。
2. **文献名称**：*Characterization of PRDM4 antibody specificity in stem cell pluripotency*
**作者**：Lee H, et al.
**摘要**：本文验证了PRDM4抗体的特异性，并发现PRDM4通过调控多能性相关基因(如OCT4)的启动子活性，维持胚胎干细胞的自我更新能力。
3. **文献名称**：*PRDM4 overexpression correlates with poor prognosis in breast cancer*
**作者**：Garcia R, et al.
**摘要**：通过免疫组化(使用PRDM4抗体)分析乳腺癌组织样本，发现PRDM4高表达与肿瘤转移及患者生存率降低显著相关。
4. **文献名称**：*PRDM4 interacts with p53 to modulate DNA damage response*
**作者**：Wang Y, et al.
**摘要**：研究利用PRDM4抗体进行免疫共沉淀(Co-IP)，证明PRDM4与p53蛋白相互作用，抑制DNA损伤修复通路，增加基因组不稳定性。
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**注意**：以上文献为示例，实际文献需通过学术数据库查询。推荐检索关键词：**PRDM4 antibody application** 或 **PRDM4 immunohistochemistry/Western blot**。
PRDM4 (PR Domain-Containing Protein 4) is a member of the PRDM family of transcriptional regulators characterized by a conserved PR (PRDI-BF1 and RIZ homology) domain, which often possesses histone methyltransferase activity. PRDM4 functions as a transcriptional repressor or activator, depending on cellular context, and is implicated in regulating cell differentiation, proliferation, and tumorigenesis. It interacts with chromatin-modifying complexes and binds to specific DNA sequences via its zinc finger motifs, influencing pathways like Wnt/β-catenin and p53. Studies link PRDM4 to neural development, stem cell maintenance, and cancer, though its role varies—acting as an oncogene in some malignancies (e.g., breast cancer) and a tumor suppressor in others (e.g., gliomas).
PRDM4 antibodies are essential tools for detecting PRDM4 expression and localization in research. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to study its expression patterns in tissues or cell lines. Commercially available antibodies target specific epitopes, such as the N-terminal PR domain or C-terminal regions. However, antibody specificity remains a challenge due to homology within the PRDM family. Researchers must validate antibodies using knockout controls or orthogonal methods. Current applications focus on elucidating PRDM4's roles in development, cancer mechanisms, and potential therapeutic targeting. Despite progress, functional studies are limited, warranting further exploration of its molecular interactions and disease associations.
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