| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GAGE7 |
| Uniprot No | O76087 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-117aa |
| 氨基酸序列 | MSWRGRSTYYWPRPRRYVQPPEMIGPMRPEQFSDEVEPATPEEGEPATQRQDPAAAQEGEDEGASAGQGPKPEAHSQEQGHPQTGCECEDGPDGQEMDPPNPEEVKTPEEGEKQSQC |
| 预测分子量 | 20.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GAGE7重组蛋白研究的3篇代表性文献示例(注：内容为模拟概括，仅供参考)：
1. **《Cloning and expression of recombinant GAGE7 protein in E. coli for cancer immunotherapy》**
- 作者：Zhang Y, et al.
- 摘要：本研究成功克隆了人源GAGE7基因，并利用大肠杆菌表达系统制备了重组GAGE7蛋白。通过亲和层析纯化获得高纯度蛋白，验证其可被癌症患者血清中的抗体特异性识别，提示其在肿瘤免疫诊断或疫苗开发中的潜在应用。
2. **《Structural characterization of GAGE7 recombinant protein reveals conserved antigenic domains》**
- 作者：Lee S, Kim JH.
- 摘要：通过X射线晶体学解析了重组GAGE7蛋白的三维结构，发现其N端含有高度保守的抗原表位区域。实验证实该重组蛋白可激活体外T细胞免疫应答，为靶向GAGE7的癌症治疗策略提供结构生物学依据。
3. **《GAGE7 recombinant protein promotes tumor cell invasion via PI3K/AKT signaling pathway》**
- 作者：Wang X, et al.
- 摘要：利用HEK293细胞表达的重组GAGE7蛋白处理多种癌细胞系，发现其通过激活PI3K/AKT信号通路显著增强肿瘤细胞的迁移和侵袭能力，提示GAGE7可能作为促癌因子参与肿瘤转移调控。
(注：实际文献需通过PubMed、Web of Science等学术平台检索确认。)
**Background of GAGE7 Recombinant Protein**
The GAGE7 (G antigen 7) protein belongs to the GAGE family of cancer/testis antigens (CTAs), a group of proteins predominantly expressed in germline cells and various malignancies but rarely in normal somatic tissues. CTAs like GAGE7 are characterized by their restricted expression pattern, making them attractive targets for cancer immunotherapy and diagnostic biomarkers. The GAGE family comprises multiple paralogs clustered on chromosome X, with GAGE7 encoding a protein containing conserved motifs involved in protein-protein interactions and potential roles in transcriptional regulation or apoptosis modulation.
GAGE7 expression is epigenetically silenced in most healthy tissues due to promoter hypermethylation but becomes reactivated in cancers such as melanoma, lung, ovarian, and hepatocellular carcinoma. This tumor-specific re-expression is linked to the hypomethylation of promoter regions during oncogenesis. Functionally, GAGE7 may contribute to tumorigenesis by promoting immune evasion, as CTAs can elicit immune responses, or by influencing cellular processes like proliferation and apoptosis. However, its exact mechanisms remain partially understood, necessitating further research.
Recombinant GAGE7 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce purified, biologically active forms for functional studies. It serves as a critical tool for investigating GAGE7's role in cancer biology, validating its interaction with immune cells (e.g., cytotoxic T lymphocytes), and developing antibody-based diagnostics or T cell-mediated therapies. Additionally, recombinant GAGE7 aids in epitope mapping and vaccine development, leveraging its immunogenic potential to target cancer cells selectively. Ongoing studies aim to clarify its prognostic value and therapeutic utility across diverse malignancies.
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