| 纯度 | >90%SDS-PAGE. |
| 种属 | E.Coli |
| 靶点 | EXPA1 |
| Uniprot No | Q7XWU8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-261aa |
| 氨基酸序列 | MAGSSAATSCARFLALLATCLLWNEAASFTASGWNKAFATFYGGSDASGTMGGACGYGDLYSTGYGTNTAALSTVLFNDGASCGQCYRIMCDYQADRRFCISGTSVTITATNLCPPNYALPNDAGGWCNPPRQHFDMAEPAWLKIGVYVGGIVPVMYQRVPCAKQGGVRFTINGRDYFELVLVSNVGGVGSIQSVSIKGSRTGWMAMSRNWGVNWQSNAYLDGQSLSFKVTSSDGQTLTFLDVAPAGWTFGQTFSTSQQFS Keywords |
| 预测分子量 | 27,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于EXPA1重组蛋白的3篇参考文献示例(注：以下内容为模拟生成，非真实文献，仅供格式参考)：
1. **文献名称**: "Expression and functional analysis of recombinant EXPA1 in Arabidopsis cell wall loosening"
**作者**: Zhang Y. et al.
**摘要**: 本研究通过大肠杆菌系统成功表达并纯化EXPA1重组蛋白，证实其可诱导拟南芥细胞壁纤维素微纤丝的解聚，在酸性条件下显著增强细胞壁延展性。
2. **文献名称**: "Optimization of EXPA1 recombinant protein production in Pichia pastoris"
**作者**: Lee S., Kim J.H.
**摘要**: 通过毕赤酵母表达系统优化EXPA1重组蛋白的分泌表达条件，获得高活性蛋白，并验证其在体外实验中促进玉米根毛细胞壁扩张的能力。
3. **文献名称**: "Crystal structure of EXPA1 reveals pH-dependent conformational changes"
**作者**: Tanaka M. et al.
**摘要**: 首次解析EXPA1重组蛋白的晶体结构，揭示其pH依赖的构象变化机制，为扩展蛋白通过质子泵作用调控细胞壁松弛提供结构生物学依据。
(注：实际文献需通过PubMed/Google Scholar等平台以关键词"EXPA1 recombinant protein"或"EXPA1 expression"检索获取。)
EXPA1 is a member of the expansin protein family, which plays a critical role in plant cell wall loosening and remodeling. Expansins are structurally conserved proteins found in plants, fungi, and bacteria, with α-expansins (like EXPA1) being specific to plants. These proteins facilitate cell wall extension by disrupting non-covalent bonds between cellulose microfibrils and matrix polysaccharides, enabling turgor-driven cell enlargement during growth, organ development, and stress responses.
The EXPA1 gene encodes a protein that is particularly associated with cell expansion in root hairs, hypocotyls, and other rapidly growing tissues. Its expression is regulated by hormonal signals (e.g., auxin, ethylene) and environmental cues. Recombinant EXPA1 protein is produced using heterologous expression systems, such as *E. coli* or yeast, to study its biochemical properties, 3D structure, and interaction with cell wall components. Purified EXPA1 retains the ability to induce wall stress relaxation *in vitro*, making it a valuable tool for investigating mechanisms of cell wall plasticity.
Research on EXPA1 has agricultural relevance, as modulating expansin activity could enhance crop resilience or biomass yield. For example, overexpression of EXPA1 in transgenic plants has been linked to improved drought tolerance and root architecture. Additionally, its potential application in biofuel production—by increasing cell wall digestibility—is under exploration. Despite progress, questions remain about its precise molecular targets and regulatory pathways, driving ongoing structural and functional studies. Overall, EXPA1 serves as a model for understanding plant cell wall dynamics and biotechnological innovations.
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