| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GUK1 |
| Uniprot No | Q16774 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-197aa |
| 氨基酸序列 | SGPRPVVLSGPSGAGKSTLLKRLLQEHSGIFGFSVSHTTRNPRPGEENGKDYYFVTREVMQRDIAAGDFIEHAEFSGNLYGTSKVAVQAVQAMNRICVLDVDLQGVRNIKATDLRPIYISVQPPSLHVLEQRLRQRNTETEESLVKRLAAAQADMESSKEPGLFDVVIINDSLDQAYAELKEALSEEIKKAQRTGA |
| 预测分子量 | 37.6kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于GUK1重组蛋白的模拟参考文献示例(请注意，实际文献需通过学术数据库检索)：
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1. **文献名称**: "Recombinant Expression and Functional Characterization of Human Guanylate Kinase 1 (GUK1) in Escherichia coli"
**作者**: Smith A., et al.
**摘要**: 本研究报道了人源GUK1基因在大肠杆菌系统中的高效重组表达及纯化。通过优化密码子使用和诱导条件，获得了可溶性活性蛋白，并验证其催化GDP转化为GMP的酶活性，为后续结构-功能研究提供基础。
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2. **文献名称**: "Structural Insights into GUK1 Dynamics: Implications for Nucleotide Binding and Neurological Disorders"
**作者**: Zhang L., et al.
**摘要**: 利用X射线晶体学解析了重组GUK1蛋白的原子结构，揭示了其核苷酸结合口袋的构象变化机制。实验发现特定突变会破坏GUK1与突触后蛋白PSD-95的相互作用，提示其在神经发育障碍中的潜在作用。
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3. **文献名称**: "GUK1 Recombinant Protein as a Therapeutic Target in Glioblastoma: Enzymatic Activity and Drug Screening"
**作者**: Patel R., et al.
**摘要**: 通过体外酶活实验验证了重组GUK1蛋白在胶质母细胞瘤细胞系中的异常高活性，并筛选了靶向GUK1的小分子抑制剂库，发现两种化合物可显著抑制肿瘤细胞增殖，为靶向治疗提供新策略。
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**建议检索途径**：
- 使用PubMed/Google Scholar，关键词：`GUK1 recombinant protein`, `Guanylate Kinase 1 expression`, `GUK1 structural analysis`
- 筛选近年文献(如2020年后)以获取最新进展。
**Background of GUK1 Recombinant Protein**
Guanylate kinase 1 (GUK1), also known as GK1. is a ubiquitously expressed enzyme that plays a critical role in nucleotide metabolism by catalyzing the reversible transfer of a phosphate group between ATP and GMP to synthesize GDP. This ATP:GMP phosphotransferase activity is essential for maintaining the balance of guanine nucleotides, which are vital for RNA/DNA synthesis, energy transfer (e.g., GTP), and cellular signaling. GUK1 is localized predominantly in the cytosol and mitochondria, linking its function to energy homeostasis and mitochondrial dynamics.
The recombinant form of GUK1 is produced using genetic engineering techniques, typically by expressing the human *GUK1* gene in bacterial (e.g., *E. coli*) or mammalian cell systems. This allows for large-scale production of the purified protein with high homogeneity, enabling structural and functional studies. Recombinant GUK1 retains its enzymatic activity and is widely used to investigate nucleotide metabolism disorders, mitochondrial dysfunction, and neurodegenerative diseases. For example, mutations in *GUK1* have been associated with developmental delays, microcephaly, and seizures, highlighting its importance in neural development.
In research, GUK1 recombinant protein serves as a tool to study enzyme kinetics, screen potential inhibitors or modulators, and explore its interaction with other proteins in nucleotide salvage pathways. Its role in cellular energetics also links it to cancer biology, where altered nucleotide metabolism is a hallmark of tumor proliferation. Furthermore, structural analyses of recombinant GUK1 via X-ray crystallography or cryo-EM have provided insights into its catalytic mechanism and regulatory domains.
Overall, GUK1 recombinant protein is a valuable resource for both basic research and therapeutic development, bridging molecular biology with clinical insights into metabolic and neurological diseases.
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