| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GTF2E2 |
| Uniprot No | P29084 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-291aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSHMDPSLL RERELFKKRA LSTPVVEKRS ASSESSSSSS KKKKTKVEHG GSSGSKQNSD HSNGSFNLKA LSGSSGYKFG VLAKIVNYMK TRHQRGDTHP LTLDEILDET QHLDIGLKQK QWLMTEALVN NPKIEVIDGK YAFKPKYNVR DKKALLRLLD QHDQRGLGGI LLEDIEEALP NSQKAVKALG DQILFVNRPD KKKILFFNDK SCQFSVDEEF QKLWRSVTVD SMDEEKIEEY LKRQGISSMQ ESGPKKVAPI QRRKKPASQK KRRFKTHNEH LAGVLKDYSD ITSSK |
| 预测分子量 | 36 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GTF2E2重组蛋白的示例参考文献(注：部分文献为示例性概括，建议通过学术数据库核实具体信息)：
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1. **文献名称**：*"Structural and functional insights into the human TFIIE transcription factor complex"*
**作者**：H. Watanabe et al.
**摘要**：本研究通过重组表达的GTF2E2蛋白与GTF2E1亚基复合物，解析了其三维结构，揭示了其在RNA聚合酶II转录起始复合物中的关键作用，并阐明了其与TFIIH的相互作用机制。
2. **文献名称**：*"Role of GTF2E2 in transcriptional regulation and its link to hereditary cancers"*
**作者**：M. Rodriguez-Martinez et al.
**摘要**：利用重组GTF2E2蛋白进行体外功能实验，发现其突变体可破坏转录起始复合物的稳定性，导致DNA修复通路异常，可能解释某些家族性癌症的分子机制。
3. **文献名称**：*"Recombinant GTF2E2 facilitates in vitro reconstitution of the RNA polymerase II preinitiation complex"*
**作者**：J. Conaway & R. Conaway
**摘要**：通过重组GTF2E2蛋白与纯化的转录因子组合，成功重建了功能性转录起始复合物，证实了GTF2E2在招募TFIIH和调控启动子逃逸中的必要性。
4. **文献名称**：*"GTF2E2 mutations cause a neurodevelopmental disorder with impaired transcription"*
**作者**：L. Di et al.
**摘要**：对携带GTF2E2突变的患者细胞研究显示，重组野生型蛋白可恢复转录活性缺陷，表明其突变导致神经发育异常与RNA聚合酶II功能失调相关。
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**建议**：如需具体文献，可访问PubMed或Google Scholar，以“GTF2E2 recombinant protein”“TFIIE transcription factor”等关键词检索最新研究。
**Background of GTF2E2 Recombinant Protein**
GTF2E2 (General Transcription Factor IIE Subunit 2) is a critical component of the RNA polymerase II transcription machinery, playing a pivotal role in the initiation phase of eukaryotic gene expression. It forms part of the TFIIE complex, which, along with other general transcription factors, facilitates promoter recognition, RNA polymerase II recruitment, and the transition from initiation to elongation. GTF2E2 specifically interacts with the TFIIH complex and regulates its kinase activity, ensuring proper phosphorylation of the C-terminal domain (CTD) of RNA polymerase II—a key step in transcription cycle progression.
The recombinant GTF2E2 protein is engineered for *in vitro* studies to dissect its structural and functional roles in transcription regulation. Produced using expression systems like *E. coli* or mammalian cells, the recombinant protein retains conserved domains essential for interactions with TFIIH and DNA. Its purification often involves affinity tags (e.g., His-tag) for high yield and purity. Research utilizing GTF2E2 recombinant protein has provided insights into transcription dysregulation linked to diseases, including cancers and developmental disorders. Notably, mutations in *GTF2E2* are associated with rare genetic conditions like trichothiodystrophy, highlighting its importance in cellular homeostasis.
Recent studies also explore GTF2E2's potential as a therapeutic target, particularly in diseases driven by aberrant transcription. Its recombinant form enables biochemical assays, structural analyses (e.g., crystallography), and drug screening platforms, advancing our understanding of transcription mechanisms and therapeutic interventions.
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