| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GTSF1 |
| Uniprot No | Q8WW33 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-167aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMEETYTD SLDPEKLLQC PYDKNHQIRA CRFPYHLIKC RKNHPDVASK LATCPFNARH QVPRAEISHH ISSCDDRSCI EQDVVNQTRS LRQETLAEST WQCPPCDEDW DKDLWEQTST PFVWGTTHYS DNNSPASNIV TEHKNNLASG MRVPKSLPYV LPWKNNGNAQ |
| 预测分子量 | 22 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于 **GTSF1(Gametocyte-Specific Transcriptional Factor 1)重组蛋白**的3篇代表性文献，包含名称、作者及摘要内容的简要概括：
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1. **文献名称**: *GTSF1 is essential for proper piRNA processing and transposon silencing in mouse germlines*
**作者**: Y. Watanabe et al.
**摘要**: 本研究阐明了GTSF1在小鼠生殖细胞中通过与PIWI蛋白互作，参与piRNA通路介导的转座子沉默。通过重组蛋白的体外实验，证实GTSF1能增强PIWI蛋白的核酸酶活性，维持基因组稳定性。基因敲除小鼠模型显示GTSF1缺失导致雄性不育及转座子异常激活。
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2. **文献名称**: *Structural and functional analysis of GTSF1 reveals its role in epigenetic regulation*
**作者**: K. Smith et al.
**摘要**: 利用X射线晶体学解析了重组GTSF1蛋白的三维结构，揭示其锌指结构域与染色质重塑复合体的结合机制。功能实验表明，GTSF1通过招募组蛋白修饰酶(如HDACs)调控生殖细胞特异性基因的表达，为生殖发育障碍提供分子机制解释。
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3. **文献名称**: *GTSF1 deficiency disrupts spermatogenesis by impairing DNA repair in germ cells*
**作者**: T. Li et al.
**摘要**: 研究报道GTSF1通过重组蛋白表达系统验证其与DNA损伤修复蛋白(如ATM/ATR)的相互作用。敲除GTSF1的小鼠精母细胞中，DNA双链断裂修复效率显著降低，导致减数分裂停滞和凋亡，提示GTSF1在生殖细胞基因组完整性中的关键作用。
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**备注**：以上文献为模拟示例，实际文献需通过PubMed、Google Scholar等平台检索确认。若需具体文献链接或补充信息，可进一步提供研究方向或关键词优化检索。
**Background of GTSF1 Recombinant Protein**
GTSF1 (Gametocyte-Specific Transcriptional Factor 1) is a conserved eukaryotic protein implicated in regulating gene expression, particularly during germline development and transposon silencing. It is best characterized for its role in the piRNA (PIWI-interacting RNA) pathway, a critical mechanism for maintaining genome stability by suppressing transposable elements in animal germ cells. Structurally, GTSF1 contains two CHHC zinc-finger domains, which mediate interactions with PIWI proteins—a class of Argonaute proteins essential for piRNA biogenesis and function.
Studies in model organisms, such as *Drosophila* and mice, reveal that GTSF1 deficiency disrupts piRNA-mediated silencing, leading to transposon activation, genomic instability, and sterility. These findings highlight its importance in fertility and epigenetic regulation. The recombinant form of GTSF1 is typically produced in *E. coli* or mammalian expression systems, enabling biochemical and functional analyses. Purification often involves affinity tags (e.g., His-tag) to ensure high yield and purity.
Research applications of GTSF1 recombinant protein include elucidating its molecular interactions with PIWI proteins, dissecting its role in piRNA complex assembly, and exploring its potential involvement in diseases linked to transposon dysregulation, such as cancer or neurodegenerative disorders. Its study also contributes to understanding broader mechanisms of RNA-guided genome defense and epigenetic inheritance.
In summary, GTSF1 recombinant protein serves as a vital tool for decoding molecular pathways essential for fertility, genome integrity, and adaptive immunity against mobile genetic elements. Its investigation bridges fundamental biology and translational research, offering insights into therapeutic strategies for related pathologies.
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