| 纯度 | >98%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GZMK |
| Uniprot No | P49863 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 27-264aa |
| 氨基酸序列 | IIGGKEVSPHSRPFMASIQYGGHHVCGGVLIDPQWVLTAAHCQYRFTKGQ SPTVVLGAHSLSKNEASKQTLEIKKFIPFSRVTSDPQSNDIMLVKLQTAA KLNKHVKMLHIRSKTSLRSGTKCKVTGWGATDPDSLRPSDTLREVTVTVL SRKLCNSQSYYNGDPFITKDMVCAGDAKGQKDSCKGDSGGPLICKGVFHA IVSGGHECGVATKPGIYTLLTKKYQTWIKSNLVPPHTN |
| 预测分子量 | 26 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GZMK重组蛋白的3篇示例文献(注：文献信息为示例性概括，非真实引用)：
1. **《Recombinant GZMK promotes apoptosis in cancer cells via caspase activation》**
- 作者：Smith J, et al.
- 摘要：研究报道了重组GZMK蛋白在体外通过激活caspase通路诱导肿瘤细胞凋亡的机制，揭示了其在抗肿瘤治疗中的潜在应用。
2. **《Expression and purification of functional human GZMK in E. coli》**
- 作者：Lee S, et al.
- 摘要：描述了一种利用大肠杆菌系统高效表达和纯化具有酶活性的重组GZMK蛋白的方法，并验证了其蛋白酶功能。
3. **《GZMK regulates immune response through cleavage of extracellular matrix proteins》**
- 作者：Wang Y, et al.
- 摘要：研究发现重组GZMK可通过切割细胞外基质蛋白(如纤维连接蛋白)调控T细胞迁移，提示其在炎症和自身免疫疾病中的作用。
(实际研究中请通过PubMed或Google Scholar检索真实文献。)
**Background of GZMK Recombinant Protein**
Granzyme K (GZMK), a serine protease primarily expressed in cytotoxic lymphocytes like natural killer (NK) cells and CD8+ T cells, plays a multifaceted role in immune responses. Unlike other granzymes (e.g., Granzyme B) that directly induce apoptosis in target cells, GZMK is associated with pro-inflammatory and non-apoptotic functions. It cleaves substrates such as extracellular matrix proteins, cytokines, and cell surface receptors, modulating immune signaling and inflammation. Studies suggest its involvement in conditions like sepsis, viral infections, and autoimmune diseases, where dysregulated inflammation or immune activation occurs.
Recombinant GZMK protein is engineered for research to elucidate its biological mechanisms and therapeutic potential. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), it retains enzymatic activity and structural integrity for *in vitro* and *in vivo* studies. Researchers use it to investigate GZMK's role in cytokine activation (e.g., IL-6. IL-8), endothelial barrier disruption, and crosstalk with other immune pathways. Its recombinant form also aids in screening inhibitors or designing targeted therapies to mitigate inflammation-related pathologies.
Despite progress, GZMK's precise physiological and pathological roles remain under exploration. Its dual role in promoting inflammation and tissue damage versus antiviral defense highlights its complexity. Recombinant GZMK thus serves as a critical tool for decoding its functions and advancing immunotherapeutic strategies.
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