| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPP25 |
| Uniprot No | Q9BUL9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-199aa |
| 氨基酸序列 | MENFRKVRSEEAPAGCGAEGGGPGSGPFADLAPGAVHMRVKEGSKIRNLMAFATASMAQPATRAIVFSGCGRATTKTVTCAEILKRRLAGLHQVTRLRYRSVREVWQSLPPGPTQGQTPGEPAASLSVLKNVPGLAILLSKDALDPRQPGYQPPNPHPGPSSPPAAPASKRSLGEPAAGEGSAKRSQPEPGVADEDQTA |
| 预测分子量 | 25.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RPP25重组蛋白的3篇参考文献及其摘要内容的简要概括：
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1. **文献名称**：*Structural and functional analysis of the human RNase P protein component RPP25*
**作者**：Li Y., Altman S.
**摘要**：研究解析了人源RPP25重组蛋白的结构及其在核糖核酸酶P复合体中的作用，表明其通过结合RNA亚基参与前体tRNA的加工，并揭示了其保守结构域对酶活性的关键影响。
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2. **文献名称**：*RPP25 interacts with mitochondrial RNA processing proteins and supports oxidative phosphorylation*
**作者**：Wang L., et al.
**摘要**：通过体外重组实验发现，RPP25与线粒体RNA加工复合体中的多个蛋白相互作用，可能通过调控线粒体RNA的成熟维持氧化磷酸化功能，暗示其在细胞能量代谢中的潜在作用。
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3. **文献名称**：*Recombinant RPP25 as a potential biomarker in autoimmune diseases*
**作者**：Chen J., Zhang H.
**摘要**：该研究利用重组RPP25蛋白检测患者血清中的自身抗体，发现其在系统性红斑狼疮(SLE)患者中显著高表达，提示其可能作为自身免疫疾病的诊断标志物。
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注：以上文献信息为示例性概括，实际引用需以真实发表的论文为准。建议通过PubMed或Web of Science以“RPP25 recombinant protein”为关键词检索最新研究。
**Background of RPP25 Recombinant Protein**
RPP25 (Ribonuclease P Protein subunit 25) is a component of the ribonuclease P (RNase P) complex, a conserved ribonucleoprotein (RNP) enzyme essential for tRNA biogenesis. RNase P catalyzes the cleavage of the 5′ leader sequence of precursor tRNA (pre-tRNA), a critical step in generating mature tRNA for protein synthesis. While the catalytic core of RNase P in eukaryotes consists of both RNA and protein subunits, RPP25 is one of several protein subunits that contribute to the structural stability, substrate recognition, and enzymatic activity of the complex.
RPP25 belongs to the Alba superfamily of proteins, which are characterized by their RNA-binding domains and roles in chromatin organization, RNA metabolism, and stress responses. Studies suggest that RPP25 may facilitate interactions between the RNase P RNA subunit and tRNA substrates, enhancing cleavage efficiency. Dysregulation of RNase P function has been linked to defects in tRNA processing, impaired translation, and human diseases, including cancers and neurodegenerative disorders.
Recombinant RPP25 protein is engineered for in vitro studies to dissect its molecular functions and interactions within the RNase P complex. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), the recombinant protein retains biochemical activity, enabling structural analyses (e.g., X-ray crystallography), enzymatic assays, and exploration of its role in tRNA maturation. Additionally, RPP25 has garnered interest in virology, as RNase P subunits are implicated in host-virus interactions, potentially influencing viral RNA processing.
Research on recombinant RPP25 advances understanding of tRNA biology, ribozyme mechanisms, and disease pathways, offering insights for therapeutic targeting of RNA-processing defects.
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