| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | MI; WS2; CMM8; WS2A; bHLHe32 |
| Entrez GeneID | 4286 |
| clone | 8F1G5 |
| WB Predicted band size | 58.8kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human MITF (AA: 1-114) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于MITF抗体的参考文献摘要概括：
1. **《MITF: a critical transcription factor in melanocyte development and melanoma》**
- **作者**: Levy C, Khaled M, Fisher DE
- **摘要**：综述了MITF在黑色素细胞分化和黑色素瘤发生中的核心作用，强调MITF抗体在免疫组化和Western blot中用于检测其表达水平及亚细胞定位，并探讨其作为治疗靶点的潜力。
2. **《Regulation of microphthalmia-associated transcription factor MITF protein levels by association with the ubiquitin-conjugating enzyme hUBC9》**
- **作者**: Xu W, Gong L, Haddad MM, et al.
- **摘要**：研究MITF蛋白稳定性调控机制，利用MITF特异性抗体通过免疫沉淀和免疫印迹技术，证明hUBC9通过泛素化途径调控MITF降解，影响黑色素瘤细胞增殖。
3. **《Lineage-specific transcriptional regulation of DICER by MITF in melanocytes》**
- **作者**: Haflidadóttir BS, Bergsteinsdóttir K, Praetorius C, et al.
- **摘要**：通过ChIP和免疫荧光实验(使用MITF抗体)，揭示MITF直接调控DICER基因表达，影响黑色素细胞发育及黑色素瘤中microRNA的生物合成。
4. **《Antibody validation for Western blot: MITF as a case study》**
- **作者**: Roncato R, Rruga F, Porcelli S, et al.
- **摘要**：系统评估不同MITF抗体的特异性与灵敏度，比较其在人黑色素瘤组织Western blot中的表现，为实验中选择可靠抗体提供依据。
以上文献均涉及MITF抗体的实验应用(如蛋白检测、机制研究或方法学验证)，覆盖基础研究与技术优化方向。
The Microphthalmia-associated transcription factor (MITF) is a key regulator of melanocyte development, differentiation, and survival. It belongs to the basic helix-loop-helix leucine zipper (bHLH-Zip) family of transcription factors and controls the expression of genes critical for melanin synthesis, such as tyrosinase (TYR), tyrosinase-related protein 1 (TYRP1), and PMEL. MITF also plays roles in other cell types, including osteoclasts, mast cells, and retinal pigment epithelium, influencing processes like bone resorption and immune response. Dysregulation of MITF is linked to diseases such as melanoma, Waardenburg syndrome (a genetic disorder affecting pigmentation and hearing), and osteoporosis.
MITF antibodies are essential tools in research and diagnostics, used to detect MITF protein expression and localization via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). These antibodies help identify MITF isoforms (e.g., MITF-M in melanocytes, MITF-A in other tissues) and assess its role in cellular pathways or disease mechanisms. In clinical settings, MITF antibodies aid in diagnosing melanocytic tumors, as MITF is a sensitive marker for melanoma. However, cross-reactivity with related transcription factors (e.g., TFE3. TFEB) requires careful validation. Studies using MITF antibodies have advanced understanding of its dual role as an oncogene and tumor suppressor, depending on cellular context, highlighting its therapeutic potential in cancer and genetic disorders.
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