| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | APG10; APG10L; pp12616 |
| Entrez GeneID | 83734 |
| clone | 8A9B3 |
| WB Predicted band size | 25.3kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human ATG10 (AA: 1-125) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于ATG10抗体的3篇参考文献示例(内容为模拟虚构，仅供参考)：
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1. **文献名称**：ATG10-mediated autophagy regulates chemoresistance in colorectal cancer
**作者**：Li, X., et al.
**摘要**：该研究通过ATG10抗体检测发现，ATG10在结直肠癌中高表达，并通过调控自噬体形成促进肿瘤细胞对化疗药物的抵抗。敲低ATG10可增强奥沙利铂的敏感性，提示其作为治疗靶点的潜力。
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2. **文献名称**：Structural insights into ATG10-ATG3 interaction during LC3 lipidation
**作者**：Tanaka, Y., et al.
**摘要**：利用ATG10抗体进行免疫共沉淀实验，揭示了ATG10与ATG3的相互作用机制，阐明了其在LC3脂化过程中的关键结构域，为自噬体形成的分子机制提供了新见解。
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3. **文献名称**：Impaired autophagy in neurodegenerative disease via ATG10 dysfunction
**作者**：Smith, J., et al.
**摘要**：研究通过ATG10抗体免疫荧光染色发现，阿尔茨海默病模型中ATG10表达异常，导致自噬通量受阻和错误蛋白堆积。恢复ATG10功能可缓解神经元损伤。
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4. **文献名称**：ATG10 antibody-based screening identifies viral inhibitors of autophagy
**作者**：Zhang, R., et al.
**摘要**：开发高特异性ATG10抗体用于高通量筛选，发现多种病毒蛋白通过干扰ATG10与ATG5-ATG12复合物的结合抑制自噬，为抗病毒策略提供新方向。
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注：以上文献信息为示例性质，实际研究需通过学术数据库(如PubMed、Web of Science)检索真实发表论文。
ATG10 (Autophagy Related 10) is a key protein involved in the autophagy pathway, a conserved cellular degradation process critical for maintaining homeostasis, nutrient recycling, and stress responses. As an E2 ubiquitin-like conjugating enzyme, ATG10 facilitates the conjugation of ATG12 to ATG5. a step essential for autophagosome formation. This interaction forms the ATG12-ATG5-ATG16L1 complex, which promotes LC3 lipidation and autophagosome membrane expansion. Dysregulation of ATG10 has been implicated in various diseases, including cancer, neurodegenerative disorders, and infections, underscoring its role in cellular health and disease pathogenesis.
ATG10 antibodies are indispensable tools for studying autophagy mechanisms. They enable the detection and quantification of ATG10 expression via techniques like Western blotting, immunofluorescence, and immunoprecipitation. Researchers use these antibodies to investigate ATG10's interaction partners, subcellular localization, and tissue-specific expression patterns. Commercially available ATG10 antibodies are typically raised in rabbits or mice, targeting specific epitopes within the protein. Validation often includes knockout cell lines or siRNA-mediated silencing to confirm specificity. Applications extend to clinical research, such as profiling ATG10 levels in tumor samples or evaluating autophagy modulation in therapeutic contexts. Proper antibody selection (monoclonal vs. polyclonal, host species, and validated applications) is critical for experimental accuracy.
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