| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | CYC; HCS; THC4 |
| Entrez GeneID | 54205 |
| clone | 2E4F8 |
| WB Predicted band size | 11.7kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CYCS (AA: 1-105) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇与CYCS(细胞色素C)抗体相关的研究文献摘要示例：
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1. **文献名称**：*Cytochrome c release and mitochondria involvement in programmed cell death induced by acetic acid in Saccharomyces cerevisiae*
**作者**：Ludovico P, et al.
**摘要**：该研究利用抗CYCS抗体通过Western blot和免疫荧光技术，证实酵母凋亡过程中细胞色素C从线粒体释放至胞质，揭示了乙酸诱导的程序性死亡与哺乳动物凋亡机制的保守性。
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2. **文献名称**：*Mitochondrial translocation of cytochrome c during apoptosis detected by a specific monoclonal antibody*
**作者**：Jiang X, et al.
**摘要**：作者开发了一种高特异性CYCS单克隆抗体，通过流式细胞术和共聚焦显微镜观察，证明在化疗药物处理的癌细胞中，细胞色素C的线粒体膜渗透性改变是凋亡早期关键事件。
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3. **文献名称**：*Impaired mitochondrial cytochrome c release in Parkinson’s disease models*
**作者**：Barsoum MJ, et al.
**摘要**：研究使用CYCS抗体对帕金森病细胞模型进行亚细胞分馏分析，发现α-突触核蛋白异常聚集会抑制细胞色素C的释放，提示线粒体功能失调在神经退行中的机制。
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**备注**：以上内容为示例，实际文献需通过PubMed或SciHub等平台检索确认。建议以“Cytochrome C antibody application”或“CYCS antibody apoptosis”为关键词查找最新研究。
CYCS antibodies target cytochrome c, a small heme-protein located in the mitochondrial intermembrane space. Cytochrome c plays a dual role in cellular processes: it is essential for the electron transport chain (ETC) as a mobile carrier transferring electrons between complexes III and IV, supporting ATP synthesis, and it acts as a key mediator in intrinsic apoptosis. During apoptosis, cytochrome c is released from mitochondria into the cytosol, where it binds Apaf-1 to form the apoptosome, triggering caspase activation and cell death.
CYCS antibodies are widely used in research to study mitochondrial function, apoptosis regulation, and diseases linked to dysregulated cell death, such as cancer, neurodegenerative disorders, and ischemia-reperfusion injury. They are employed in techniques like Western blotting, immunohistochemistry, and flow cytometry to detect cytochrome c localization, expression levels, or release dynamics. Mutations in the CYCS gene, though rare, have been associated with thrombocytopenia and mitochondrial disorders, making these antibodies valuable for diagnostic and mechanistic studies.
As cytochrome c's role spans metabolism and apoptosis, CYCS antibodies serve as critical tools for exploring cellular stress responses, drug-induced toxicity, and therapeutic strategies targeting mitochondrial pathways.
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