| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | BRPK; PARK6 |
| Entrez GeneID | 65018 |
| clone | 5F2B11 |
| WB Predicted band size | 62.8kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human PINK1 (AA: 112-273) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于PINK1抗体的3-4篇参考文献及其摘要概括：
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1. **"PINK1 stabilized by mitochondrial depolarization recruits Parkin to damaged mitochondria and activates latent Parkin for mitophagy"**
*Matsuda, N., et al. (Nature, 2010)*
该研究通过使用特异性PINK1抗体，证实PINK1在受损线粒体膜电位下降时积累，并招募Parkin蛋白启动线粒体自噬。抗体被用于免疫印迹和免疫荧光，验证PINK1在线粒体损伤后的表达定位。
2. **"Comparative analysis of PINK1 antibodies for the detection of endogenous PINK1 in human cells"**
*Vives-Bauza, C., et al. (PLoS ONE, 2011)*
研究系统性评估了多种市售PINK1抗体的特异性及灵敏度，发现部分抗体在特定实验条件(如蛋白酶抑制)下能有效检测内源性PINK1.为抗体选择提供了实验依据。
3. **"The ubiquitin kinase PINK1 recruits autophagy receptors to induce mitophagy"**
*Lazarou, M., et al. (Nature, 2015)*
利用构象特异性PINK1抗体，揭示了PINK1激酶活性依赖的构象变化对其功能的关键作用，抗体被用于免疫沉淀及活细胞成像，解析PINK1在自噬受体招募中的机制。
4. **"PINK1 is activated by mitochondrial membrane potential loss and recruits LC3 to damaged mitochondria"**
*Yamano, K., & Youle, R.J. (Journal of Cell Biology, 2015)*
通过Western blot和免疫染色验证PINK1抗体，证明线粒体损伤后PINK1通过磷酸化泛素激活Parkin，并依赖抗体检测PINK1的切割形式以研究其激活动力学。
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这些文献涵盖了PINK1抗体的应用场景(如功能研究、抗体验证、动态检测等)，为相关实验设计提供了参考。
PINK1 (PTEN-induced putative kinase 1) is a serine/threonine kinase primarily localized to mitochondria, playing a critical role in mitochondrial quality control and mitophagy. It is encoded by the *PARK6* gene, and mutations in PINK1 are linked to autosomal recessive early-onset Parkinson’s disease (PD). Under normal conditions, PINK1 is imported into healthy mitochondria and degraded. However, during mitochondrial stress or damage, PINK1 accumulates on the outer membrane, where it phosphorylates ubiquitin and the E3 ligase Parkin, initiating a cascade to tag damaged mitochondria for autophagic clearance. This pathway is vital for maintaining mitochondrial homeostasis and neuronal survival.
PINK1 antibodies are essential tools for studying its expression, localization, and function in both physiological and disease contexts. These antibodies enable detection of PINK1 in techniques like Western blotting, immunofluorescence, and immunohistochemistry, helping researchers assess its levels in cellular models of PD or other neurodegenerative disorders. Specific antibodies may target distinct regions of PINK1. such as the N-terminus or kinase domain, aiding in differentiating full-length proteins from cleaved fragments. Commercial PINK1 antibodies are often validated in knockout models to ensure specificity. Their application has advanced understanding of mitochondrial dysfunction mechanisms in PD, contributing to therapeutic development targeting mitophagy pathways. However, variability in antibody performance across experimental conditions requires careful optimization to ensure reliable results.
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