| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | JNK2; SAPK; p54a; JNK2A; JNK2B; PRKM9; JNK-55; SAPK1a; JNK2BETA; p54aSAPK; JNK2ALPHA |
| Entrez GeneID | 5601 |
| clone | 2F6H11 |
| WB Predicted band size | 48.1kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human MAPK9 (AA: 227-382) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
+ +
以下是关于MAPK9抗体的3篇参考文献及其简要摘要：
---
1. **文献名称**：*"Selective roles of JNK2 in spatial memory and synaptic plasticity"*
**作者**：Sherrin T. et al.
**摘要**：本研究利用MAPK9(JNK2)特异性抗体进行Western blot和免疫组化，发现JNK2在小鼠海马区的突触可塑性和空间记忆形成中具有关键作用，抗体特异性验证排除了JNK1的交叉反应。
---
2. **文献名称**：*"MAPK9-mediated phosphorylation in diabetic nephropathy progression"*
**作者**：Yang Y. et al.
**摘要**：通过MAPK9抗体检测肾组织样本中的蛋白表达及磷酸化水平，研究揭示MAPK9信号在糖尿病肾病中的异常激活，抗体应用于免疫沉淀技术以分析其与下游靶点的相互作用。
---
3. **文献名称**：*"Antibody-based inhibition of JNK2 suppresses tumor growth in colorectal cancer models"*
**作者**：Chen L. et al.
**摘要**：开发了一种高特异性MAPK9单克隆抗体，并在结直肠癌模型中验证其抑制肿瘤增殖的效果。研究通过流式细胞术和免疫荧光证实抗体可阻断JNK2的核转位。
---
这些文献均明确涉及MAPK9抗体的实验应用(如蛋白检测、定位或功能抑制)，涵盖神经科学、疾病机制及治疗开发方向。
The MAPK9 antibody targets the Mitogen-Activated Protein Kinase 9 (MAPK9), also known as c-Jun N-terminal kinase 2 (JNK2), a member of the serine/threonine kinase family. MAPK9 is part of the JNK signaling pathway, which responds to extracellular stimuli such as cytokines, stress signals (e.g., UV radiation, osmotic shock), and growth factors. It regulates diverse cellular processes, including apoptosis, differentiation, proliferation, and inflammation. Upon activation via phosphorylation by upstream kinases (MAP2Ks), MAPK9 translocates to the nucleus, where it phosphorylates transcription factors like c-Jun, ATF2. and p53. modulating gene expression. Dysregulation of MAPK9 is implicated in diseases such as cancer, neurodegenerative disorders, and inflammatory conditions.
MAPK9 antibodies are critical tools for detecting and quantifying MAPK9 protein levels, phosphorylation status, and subcellular localization in research. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. Antibodies may vary in clonality (monoclonal/polyoclonal), species reactivity (human, mouse, rat), and specificity (phospho-specific vs. total protein). Validating antibody specificity is essential to avoid cross-reactivity with closely related isoforms (e.g., JNK1 or JNK3). Applications include studying stress response pathways, disease mechanisms, and evaluating therapeutic agents targeting JNK signaling. Commercial MAPK9 antibodies often cite peer-reviewed data to ensure reproducibility in experimental models, from cell lines to tissue samples.
×
