| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200-1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200-1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | FLJ36605; VIM |
| Entrez GeneID | 7431 |
| clone | 3D2F9 |
| WB Predicted band size | 53.7kDa |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Purified recombinant fragment of human VIM (AA: 2-466) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3条关于Vimentin抗体的参考文献示例(文献信息为模拟概括，实际引用时需核实原文)：
1. **文献名称**: *Vimentin: Structure, Function, and Its Role in Cell Migration*
**作者**: Sarrai M, et al.
**摘要**: 综述了Vimentin作为中间丝蛋白的结构特征及其在维持细胞完整性、细胞迁移和信号传导中的作用，重点讨论了Vimentin抗体在细胞骨架动态研究中的应用。
2. **文献名称**: *Vimentin as a Marker for Mesenchymal Differentiation in Colorectal Cancer*
**作者**: Kokkinos MI, et al.
**摘要**: 通过免疫组化分析，研究Vimentin在结直肠癌中的表达，证明其作为上皮-间充质转化(EMT)标志物的价值，并探讨Vimentin抗体在癌症转移诊断中的潜力。
3. **文献名称**: *Antibody-Based Inhibition of Vimentin Disrupts Cellular Mechanosensing*
**作者**: Fillmore HL, et al.
**摘要**: 利用特异性Vimentin抗体阻断其功能，发现Vimentin在细胞机械信号感知和干细胞分化中的关键作用，为靶向干预提供实验依据。
如需具体文献，建议通过PubMed或Google Scholar检索关键词“Vimentin antibody” + 研究领域(如“cancer”“EMT”等)。
Vimentin is a type III intermediate filament protein predominantly expressed in mesenchymal cells, playing a critical role in maintaining cell integrity, regulating adhesion, migration, and intracellular signaling. As a marker of mesenchymal differentiation, vimentin is widely used in research to identify fibroblasts, endothelial cells, and certain cancer cells undergoing epithelial-mesenchymal transition (EMT), a process linked to metastasis and tumor progression.
Vimentin antibodies are essential tools in studying cellular dynamics in both physiological and pathological contexts. In research, they are employed in techniques like immunofluorescence, immunohistochemistry (IHC), and Western blotting to visualize vimentin expression patterns in tissues or cultured cells. These antibodies help investigate conditions such as fibrosis, wound healing, and cancer, where altered vimentin expression correlates with disease severity or prognosis. For instance, elevated vimentin levels in carcinomas often indicate EMT activation, associated with aggressive tumor behavior.
Commercially available vimentin antibodies are typically raised against conserved epitopes, ensuring cross-reactivity across species like human, mouse, and rat. Monoclonal antibodies offer high specificity, while polyclonal versions may detect multiple isoforms. Validation includes testing in knockout models or recombinant protein assays to confirm specificity. Researchers must optimize protocols (e.g., antigen retrieval in IHC) to avoid cross-reactivity with other intermediate filaments. Overall, vimentin antibodies remain indispensable for exploring cytoskeletal dynamics and disease mechanisms in biomedical studies.
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