| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HSD17B8 |
| Uniprot No | Q92506 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-261aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MASQLQNRLR SALALVTGAG SGIGRAVSVR LAGEGATVAA CDLDRAAAQE TVRLLGGPGS KEGPPRGNHA AFQADVSEAR AARCLLEQVQ ACFSRPPSVV VSCAGITQDE FLLHMSEDDW DKVIAVNLKG TFLVTQAAAQ ALVSNGCRGS IINISSIVGK VGNVGQTNYA ASKAGVIGLT QTAARELGRH GIRCNSVLPG FIATPMTQKV PQKVVDKITE MIPMGHLGDP EDVADVVAFL ASEDSGYITG TSVEVTGGLF M |
| 预测分子量 | 29 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HSD17B8重组蛋白的3篇代表性文献示例(注：文献信息为模拟生成，供参考)：
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1. **文献名称**：*Cloning, expression, and characterization of recombinant human HSD17B8: A novel short-chain dehydrogenase involved in steroid metabolism*
**作者**：Smith A, et al.
**摘要**：本研究成功克隆并表达了人源HSD17B8基因的重组蛋白，通过大肠杆菌表达系统获得高纯度蛋白。酶活性分析表明，HSD17B8对雌酮和睾酮等性激素前体具有特异性还原酶活性，提示其在类固醇激素代谢中的潜在作用。
2. **文献名称**：*Structural insights into HSD17B8 catalysis: X-ray crystallography and mutational analysis*
**作者**：Tanaka K, et al.
**摘要**：通过X射线晶体学解析了HSD17B8的三维结构(分辨率2.1 Å)，揭示了其NADPH结合口袋和底物识别关键残基。突变实验证实了His-156和Asp-169在催化中的核心作用，为设计靶向抑制剂提供结构基础。
3. **文献名称**：*HSD17B8 overexpression in breast cancer cells modulates estrogen receptor signaling*
**作者**：Wang L, et al.
**摘要**：研究发现，HSD17B8重组蛋白在乳腺癌细胞中过表达可促进雌二醇生成，增强雌激素受体(ER)信号通路活性，提示HSD17B8可能通过局部激素合成参与乳腺癌进展。
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如需真实文献，建议通过PubMed或Web of Science以“HSD17B8”和“recombinant protein”为关键词检索。
**Background of HSD17B8 Recombinant Protein**
HSD17B8 (17β-hydroxysteroid dehydrogenase type 8) is a member of the 17β-HSD enzyme family, which plays critical roles in steroid hormone metabolism by catalyzing the oxidation or reduction of hydroxyl/keto groups in steroids. Specifically, HSD17B8 is involved in the conversion of estrone (E1) to estradiol (E2), a key estrogen hormone, and contributes to the regulation of steroid-dependent physiological processes. Beyond steroid metabolism, HSD17B8 exhibits multifunctional activity, participating in fatty acid biosynthesis and neurotransmitter metabolism, highlighting its broad biological relevance.
The recombinant HSD17B8 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cell lines) to enable large-scale production for research and therapeutic applications. Its recombinant form retains enzymatic activity, allowing studies on substrate specificity, catalytic mechanisms, and interactions with inhibitors or modulators. Structural analyses reveal that HSD17B8 adopts a short-chain dehydrogenase/reductase (SDR) fold, with conserved NAD⁺/NADH-binding motifs critical for its redox function.
Research on recombinant HSD17B8 has implications for understanding diseases linked to hormonal dysregulation, such as breast cancer, endometriosis, and metabolic disorders. It also serves as a tool for drug discovery, particularly in targeting estrogen-dependent pathologies. Additionally, studies suggest HSD17B8's role in neurological health, potentially linking it to neurodegenerative conditions.
Quality-controlled recombinant HSD17B8 is typically purified via affinity chromatography and validated using SDS-PAGE, western blotting, and activity assays. Its stability and functional consistency make it a valuable reagent for both *in vitro* and *in vivo* investigations into steroid biology and metabolic pathways.
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