| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HSPB9 |
| Uniprot No | Q9BQS6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-159aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMQRVGNTFSNESRVASRCPSVGLAERNRVA TMPVRLLRDSPAAQEDNDHARDGFQMKLDAHGFAPEELVVQVDGQWLMVT GQQQLDVRDPERVSYRMSQKVHRKMLPSNLSPTAMTCCLTPSGQLWVRGQ CVALALPEAQTGPSPRLGSLGSKASNLTR |
| 预测分子量 | 20 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HSPB9重组蛋白的3篇参考文献的示例(注：文献信息为假设性概括，实际需根据真实文献调整)：
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1. **文献名称**: *"Recombinant HSPB9 Expression in E. coli and Its Role in Sperm Oxidative Stress Resistance"*
**作者**: Zhang L, et al.
**摘要**: 本研究成功在大肠杆菌中表达并纯化了HSPB9重组蛋白，发现其能显著增强精子在氧化应激条件下的存活率，提示HSPB9可能在男性生殖系统中发挥抗氧化保护作用。
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2. **文献名称**: *"Structural Characterization of HSPB9 Recombinant Protein and Its Chaperone Activity"*
**作者**: Tanaka K, et al.
**摘要**: 通过X射线晶体学解析了HSPB9重组蛋白的三维结构，证明其具备典型的小热休克蛋白α-晶状体结构域。体外实验表明，HSPB9能抑制热诱导的蛋白质聚集，具有分子伴侣功能。
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3. **文献名称**: *"HSPB9 Recombinant Protein Suppresses Apoptosis in Cancer Cells via Regulating Bcl-2/Bax Pathway"*
**作者**: Wang Y, et al.
**摘要**: 研究发现，HSPB9重组蛋白在体外显著抑制化疗药物诱导的肿瘤细胞凋亡，其机制可能与调控Bcl-2/Bax蛋白平衡及线粒体膜电位稳定性有关。
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**注意**：以上文献为示例，实际引用需检索PubMed、Web of Science等数据库获取真实文献。若需具体文献，建议使用关键词“HSPB9 recombinant protein”或“HSPB9 expression and function”进一步检索。
HSPB9. also known as heat shock protein beta-9. is a member of the small heat shock protein (sHSP) family characterized by a conserved α-crystallin domain. These proteins function as molecular chaperones, assisting in protein folding, preventing aggregation under stress conditions, and maintaining cellular homeostasis. While HSPB9 shares structural similarities with other sHSPs like HSPB1 and HSPB5. its expression profile and biological roles remain less characterized. It is predominantly expressed in the testis, suggesting a potential role in spermatogenesis or germ cell protection, though its precise mechanism remains unclear.
Recombinant HSPB9 refers to the artificially produced version of the protein using expression systems such as *E. coli*, yeast, or mammalian cells. This approach enables controlled production of the protein with high purity for functional studies. Researchers often clone the HSPB9 gene into expression vectors, followed by transfection into host cells, induction of protein synthesis, and purification via affinity chromatography (e.g., His-tag systems). Recombinant HSPB9 retains chaperone activity, making it valuable for investigating its interaction with client proteins, stress response pathways, and structural dynamics.
Interest in HSPB9 has grown due to emerging links to reproductive health and disease. Studies suggest its involvement in mitigating oxidative stress in male germ cells, potentially influencing fertility. Additionally, aberrant expression has been observed in certain cancers, though its role in oncogenesis remains speculative. The availability of recombinant HSPB9 facilitates *in vitro* assays, antibody development, and structural studies (e.g., crystallography or cryo-EM) to elucidate its functional domains. Challenges include optimizing solubility and stability, as sHSPs often form dynamic oligomers. Further research using recombinant tools may clarify HSPB9's physiological significance and therapeutic potential in stress-related pathologies.
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