| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HSPB7 |
| Uniprot No | Q9UBY9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-170aa |
| 氨基酸序列 | MSHRTSSTFRAERSFHSSSSSSSSSTSSSASRALPAQDPPMEKALSMFSD DFGSFMRPHSEPLAFPARPGGAGNIKTLGDAYEFAVDVRDFSPEDIIVTT SNNHIEVRAEKLAADGTVMNTFAHKCQLPEDVDPTSVTSALREDGSLTIR ARRHPHTEHVQQTFRTEIKILEHHHHHH |
| 预测分子量 | 19 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HSPB7重组蛋白的3篇参考文献示例(注：文献为虚拟示例，实际引用需核实真实来源)：
1. **文献名称**：*Recombinant HSPB7 suppresses oxidative stress-induced apoptosis in cardiomyocytes*
**作者**：Liu Y, et al.
**摘要**：研究通过在大肠杆菌中表达重组HSPB7蛋白，验证其在心肌细胞中抑制氧化应激诱导的细胞凋亡，揭示其通过调节Bcl-2/Bax通路发挥保护作用。
2. **文献名称**：*Structural characterization of human HSPB7 using recombinant expression and cryo-EM*
**作者**：Smith J, et al.
**摘要**：利用重组技术表达人源HSPB7.通过冷冻电镜解析其三维结构，发现其寡聚化模式与心肌细胞中应激响应相关。
3. **文献名称**：*HSPB7 recombinant protein ameliorates cardiac fibrosis in diabetic mice*
**作者**：Chen X, et al.
**摘要**：在小鼠模型中注射重组HSPB7蛋白，发现其通过抑制TGF-β/Smad信号通路显著减轻糖尿病引发的心脏纤维化。
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若需真实文献，建议通过PubMed或Google Scholar检索关键词“HSPB7 recombinant protein”或结合具体研究领域(如心脏疾病)筛选。
HSPB7 (Heat Shock Protein Family B Member 7), also known as cardiovascular heat shock protein (cvHSP), is a member of the small heat shock protein (sHSP) family characterized by a conserved α-crystallin domain. Unlike other sHSPs, HSPB7 lacks the typical chaperone-like activity for preventing protein aggregation under stress but plays distinct roles in cellular homeostasis. It is highly expressed in cardiac and skeletal muscle tissues, suggesting its importance in maintaining muscle integrity and function. Studies link HSPB7 to cardiovascular diseases, including cardiomyopathy and heart failure, as well as metabolic disorders like diabetes. Its involvement in regulating apoptosis, autophagy, and cytoskeletal organization highlights its multifaceted role in cellular stress responses.
Recombinant HSPB7 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to produce purified, biologically active forms for research. This tool enables precise investigation of HSPB7’s molecular mechanisms, such as its interaction with titin (a giant muscle protein) or desmin filaments, which are critical for sarcomere structure. Researchers also utilize recombinant HSPB7 to explore its paradoxical roles—while its deficiency exacerbates cardiac dysfunction, overexpression may inhibit protective stress responses. Additionally, it aids in studying post-translational modifications (e.g., phosphorylation) that modulate its function. As a potential therapeutic target, recombinant HSPB7 underpins drug discovery efforts aimed at mitigating muscle atrophy, cardiac hypertrophy, or metabolic syndromes. Its unique tissue-specific expression and functional complexity make it a compelling subject for both basic and translational research.
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